Faculty of Environmental Science and Engineering, 47910Kunming University of Science and Technology, Kunming, Yunnan, China.
Department of Gastroenterology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China.
Technol Cancer Res Treat. 2022 Jan-Dec;21:15330338221118717. doi: 10.1177/15330338221118717.
Forkhead box F2, a member of the Forkhead box transcription factor superfamily, plays an important role in several types of cancer. However, the mechanisms of Forkhead box F2 in the progression of colorectal cancer remain unclear. PRUNE2 is closely associated with prostate cancer, neuroblastoma, glioblastoma, and melanoma. The relationship between Forkhead box F2 and PRUNE2 in colorectal cancer remains unknown. We investigated the effects of Forkhead box F2 upregulation on colorectal cancer cell behavior using Cell Counting Kit-8, colony formation, flow cytometry, Transwell, reverse transcription quantitative polymerase chain reaction and Western blot analyses. Nude mouse xenografts were established to investigate the effect of Forkhead box F2 upregulation on the growth of colorectal cancer cells. Dual-luciferase reporter assays were performed to confirm the Forkhead box F2 regulation of PRUNE2 transcription. A series of assays was performed in cells with Forkhead box F2 upregulation and PRUNE2 knockdown to elucidate the function and regulatory effects of Forkhead box F2 on PRUNE2 transcription in colorectal cancer. Forkhead box F2 was downregulated in colorectal cancer tissues compared with adjacent tissues. Forkhead box F2 overexpression significantly suppressed the proliferation and invasion of colorectal cancer cells and . Moreover, Forkhead box F2 directly targeted PRUNE2 to promote its transcription in colorectal cancer cells. Furthermore, PRUNE2 mediated the Forkhead box F2-regulated proliferation and invasion of colorectal cancer cells. Additionally, we demonstrated a significant positive correlation between Forkhead box F2 and PRUNE2 mRNA levels in colorectal cancer tissues. These results indicated that Forkhead box F2 and PRUNE2 in combination may serve as a prognostic biomarker for colorectal cancer and that Forkhead box F2 upregulation inhibits the proliferation and invasion of colorectal cancer cells by upregulating PRUNE2.
叉头框转录因子 F2（Forkhead box F2，FoxF2）是叉头框转录因子超家族的成员之一，在多种类型的癌症中发挥着重要作用。然而，FoxF2 在结直肠癌进展中的作用机制尚不清楚。PRUNE2 与前列腺癌、神经母细胞瘤、胶质母细胞瘤和黑色素瘤密切相关。FoxF2 与结直肠癌中 PRUNE2 的关系尚不清楚。我们使用细胞计数试剂盒-8、集落形成、流式细胞术、Transwell 实验、逆转录定量聚合酶链反应和 Western blot 分析来研究 FoxF2 上调对结直肠癌细胞行为的影响。建立裸鼠异种移植模型来研究 FoxF2 上调对结直肠癌细胞生长的影响。双荧光素酶报告基因实验来验证 FoxF2 对 PRUNE2 转录的调控作用。在 FoxF2 上调和 PRUNE2 敲低的细胞中进行一系列实验，以阐明 FoxF2 对结直肠癌中 PRUNE2 转录的功能和调控作用。FoxF2 在结直肠癌组织中的表达水平低于相邻组织。FoxF2 过表达显著抑制结直肠癌细胞的增殖和侵袭。此外，FoxF2 直接靶向 PRUNE2 以促进其在结直肠癌细胞中的转录。此外，PRUNE2 介导了 FoxF2 调节的结直肠癌细胞的增殖和侵袭。此外，我们还证明了 FoxF2 和 PRUNE2 在结直肠癌组织中的 mRNA 水平之间存在显著的正相关。这些结果表明，FoxF2 和 PRUNE2 联合可能作为结直肠癌的预后生物标志物，FoxF2 上调通过上调 PRUNE2 抑制结直肠癌细胞的增殖和侵袭。
