School of Dentistry, The University of Queensland, Herston, QLD 4006, Australia.
School of Mechanical and Mining Engineering, The University of Queensland, St Lucia, QLD 4072, Australia.
Biomater Adv. 2022 Apr;135:212748. doi: 10.1016/j.bioadv.2022.212748. Epub 2022 Mar 17.
Incorporation of a bioactive mineral filler in a biodegradable polyester scaffold is a promising strategy for scaffold assisted bone tissue engineering (TE). The current study evaluates the in vitro behavior of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV)/Akermanite (AKM) composite scaffolds manufactured using selective laser sintering (SLS). Exposure of the mineral filler on the surface of the scaffold skeleton was evident from in vitro mineralization in PBS. PHBV scaffolds and solvent cast films served as control samples and all materials showed preferential adsorption of fibronectin compared to serum albumin as well as non-cytotoxic response in human osteoblasts (hOB) at 24 h. hOB culture for up to 21 days revealed that the metabolic activity in PHBV films and scaffolds was significantly higher than that of PHBV/AKM scaffolds within the first two weeks of incubation. Afterwards, the metabolic activity in PHBV/AKM scaffolds exceeded that of the control samples. Confocal imaging showed cell penetration into the porous scaffolds. Significantly higher ALP activity was observed in PHBV/AKM scaffolds at all time points in both basal and osteogenic media. Mineralization during cell culture was observed on all samples with PHBV/AKM scaffolds exhibiting distinctly different mineral morphology. This study has demonstrated that the bioactivity of PHBV SLS scaffolds can be enhanced by incorporating AKM, making this an attractive candidate for bone TE application.
在可生物降解聚酯支架中掺入生物活性矿物填料是支架辅助骨组织工程（TE）的一种很有前途的策略。本研究评估了使用选择性激光烧结（SLS）制造的聚（羟基丁酸酯-共-羟基戊酸酯）（PHBV）/硅灰石（AKM）复合支架的体外行为。从 PBS 中的体外矿化可以明显看出，矿物填料暴露在支架骨架的表面上。PHBV 支架和溶剂浇铸薄膜作为对照样品，所有材料在 24 小时内均表现出对纤维连接蛋白的优先吸附，而不是对血清白蛋白的吸附，以及对人成骨细胞（hOB）的非细胞毒性反应。hOB 培养长达 21 天表明，在孵育的头两周内，PHBV 薄膜和支架的代谢活性明显高于 PHBV/AKM 支架。之后，PHBV/AKM 支架的代谢活性超过了对照样品。共聚焦成像显示细胞渗透到多孔支架中。在基础和成骨培养基中，在所有时间点，PHBV/AKM 支架的碱性磷酸酶（ALP）活性均明显更高。在所有样品中都观察到细胞培养过程中的矿化，而 PHBV/AKM 支架表现出明显不同的矿物质形态。这项研究表明，通过掺入 AKM 可以增强 PHBV SLS 支架的生物活性，使其成为骨 TE 应用的有吸引力的候选者。
