Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
Virology. 2022 Sep;574:65-70. doi: 10.1016/j.virol.2022.07.005. Epub 2022 Jul 21.
Although not critical for hepatitis B virus (HBV) replication, splicing of HBV pre-genomic RNA generates multiple HBV splice variants, some of which have been shown to impact replication of the genome-length HBV on which they rely for their replication. To date, all replication studies of splice variants have utilised truncated RNA or over-expression constructs, and studies utilising constructs that produce authentic splice derived HBV RNA are lacking. Here we utilise a greater than genome length model to interrogate the complete replication phenotype of HBV splice variant Sp1, and investigate mechanisms by which it negatively impacts genome-length HBV replication.
虽然乙型肝炎病毒 (HBV) 前基因组 RNA 的剪接对于 HBV 的复制不是关键的，但它会产生多种 HBV 剪接变体，其中一些已被证明会影响其依赖的全长 HBV 的复制。迄今为止，所有剪接变体的复制研究都利用了截断的 RNA 或过表达构建体，而缺乏利用产生真正剪接衍生 HBV RNA 的构建体的研究。在这里，我们利用一个大于基因组长度的模型来研究 HBV 剪接变体 Sp1 的完整复制表型，并研究其负向影响全长 HBV 复制的机制。
