Ucci G, Mazzini G, Caporali R, Danova M, Montecucco C M, Riccardi A
Basic Appl Histochem. 1987;31(1):83-90.
The nuclear DNA content distribution of peripheral blood (PB) and bone marrow (BM) cells was determined by propidium iodide flow cytometry in 33 patients who underwent BM aspiration for diagnostic purposes. Two types of BM samples were taken during every aspiration procedure: whole BM aspirate, composed of BM particles contaminated by PB cells; isolated BM particles. Proliferative activity was calculated as the percentage of cells with DNA content intermediate between the diploid (2n) and the tetraploid (4n) values (2n-4n%). Ploidy was expressed as the ratio between the modal channel of the G0-G1 peak of the probe and that of an internal reference standard (DNA index, DI). The 2n-4n% was very close to zero in all PB samples. It was significantly greater in BM particles (21.2 +/- 6.6%) than in whole BM aspirate (16.6 +/- 5.5%, p less than .0005), with a close correlation (r2 = 66; p less than .0001) between the two values. Aneuploid stem lines were found in BM but not in PB. The DI of BM stem lines were similar in whole BM aspirate and BM particles, but the percentage of aneuploid cells was usually higher in BM particles. The reduced proliferative activity and the lower percent of aneuploid cells found in whole BM aspirates, with respect to BM particles, can be attributed to the contamination of BM tissue by PB, which had a very low proliferative activity and did not show aneuploidy. BM particles are therefore an easily obtained and reliable sample for routine evaluation of proliferative activity and ploidy of BM cells by DNA flow cytometry.
对33例因诊断目的而行骨髓穿刺的患者，采用碘化丙啶流式细胞术测定其外周血（PB）和骨髓（BM）细胞的核DNA含量分布。每次穿刺过程中采集两种类型的骨髓样本：全骨髓抽吸物，由被PB细胞污染的BM颗粒组成；分离的BM颗粒。增殖活性以DNA含量介于二倍体（2n）和四倍体（4n）值之间的细胞百分比（2n - 4n%）计算。倍性表示为探针G0 - G1峰的模态通道与内部参考标准（DNA指数，DI）的模态通道之比。所有PB样本中的2n - 4n%非常接近零。BM颗粒中的该值（21.2 +/- 6.6%）显著高于全骨髓抽吸物（16.6 +/- 5.5%，p <.0005），两者之间具有密切相关性（r2 = 66；p <.0001）。在BM中发现了非整倍体干细胞系，但在PB中未发现。全骨髓抽吸物和BM颗粒中BM干细胞系的DI相似，但BM颗粒中非整倍体细胞的百分比通常更高。相对于BM颗粒，全骨髓抽吸物中增殖活性降低和非整倍体细胞百分比降低可归因于PB对BM组织的污染，PB的增殖活性非常低且未显示非整倍性。因此，BM颗粒是通过DNA流式细胞术对BM细胞的增殖活性和倍性进行常规评估的一种易于获取且可靠的样本。
