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低氯介质中红细胞钾离子外流增加所涉及的因素。

Factors involved in the increase of K+ efflux of erythrocytes in low chloride media.

作者信息

Bernhardt I, Erdmann A, Vogel R, Glaser R

出版信息

Biomed Biochim Acta. 1987;46(2-3):S36-40.

PMID:3593314
Abstract

The rate constant of the Rb+(K+) efflux in isotonic NaCl solutions containing sucrose is increased when the NaCl concentration is reduced. When NaCl is replaced by Na-gluconate no change of the rate constant can be observed. The ionic strength of the solution is discussed as one factor influencing the Rb+(K+) efflux. The addition of furosemide (0.5 mM) inhibits the rate constant of the Rb+(K+) efflux in solutions of different ionic strength by about 50%. The increase of the rate constant in solutions of low ionic strength containing sucrose is interpreted as a stimulation of the chloride-dependent Na,K cotransport and the K,Cl cotransport.

摘要

在含有蔗糖的等渗氯化钠溶液中,当氯化钠浓度降低时,铷离子(钾离子)外流的速率常数会增加。当用葡萄糖酸钠替代氯化钠时,未观察到速率常数的变化。溶液的离子强度被视为影响铷离子(钾离子)外流的一个因素。添加呋塞米(0.5 mM)可使不同离子强度溶液中铷离子(钾离子)外流的速率常数降低约50%。在含有蔗糖的低离子强度溶液中速率常数的增加被解释为对氯离子依赖性钠钾协同转运和钾氯协同转运的刺激。

相似文献

1
Factors involved in the increase of K+ efflux of erythrocytes in low chloride media.低氯介质中红细胞钾离子外流增加所涉及的因素。
Biomed Biochim Acta. 1987;46(2-3):S36-40.
2
Species-dependent differences in the influence of ionic strength on potassium transport of erythrocytes. The role of membrane fluidity and Ca2+.离子强度对红细胞钾转运影响的物种依赖性差异。膜流动性和Ca2+的作用。
Gen Physiol Biophys. 1990 Dec;9(6):577-88.
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Species-dependent differences in the effect of ionic strength on potassium transport of erythrocytes: the role of lipid composition.离子强度对红细胞钾转运影响的种属依赖性差异:脂质组成的作用。
Gen Physiol Biophys. 1992 Jun;11(3):287-99.
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Furosemide-sensitive K+ (Rb+) transport in human erythrocytes: modes of operation, dependence on extracellular and intracellular Na+, kinetics, pH dependency and the effect of cell volume and N-ethylmaleimide.速尿敏感的人红细胞钾(铷)转运:运转模式、对细胞外和细胞内钠离子的依赖性、动力学、pH依赖性以及细胞体积和N-乙基马来酰亚胺的影响
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Effects of charged amphiphiles in depolarising solutions on potassium efflux and the osmotic fragility of human erythrocytes.去极化溶液中带电两亲分子对人红细胞钾外流和渗透脆性的影响。
Bioelectrochemistry. 2008 Aug;73(2):117-22. doi: 10.1016/j.bioelechem.2008.04.001. Epub 2008 Apr 11.
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Intracellular sodium, potassium and magnesium concentration, ouabain-sensitive 86rubidium-uptake and sodium-efflux and Na+, K+-cotransport activity in erythrocytes of normal male subjects studied on two occasions.对正常男性受试者的红细胞进行了两次研究,测定了细胞内钠、钾和镁的浓度、哇巴因敏感的86铷摄取、钠外流以及钠钾协同转运活性。
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Inhibition and stimulation of K+ transport across the frog erythrocyte membrane by furosemide, DIOA, DIDS and quinine.速尿、二异丁基乙酰胺(DIOA)、4,4'-二异硫氰基芪-2,2'-二磺酸(DIDS)和奎宁对青蛙红细胞膜钾离子转运的抑制和刺激作用。
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Investigations on the control of ion transport in human erythrocytes. I. Passive 86Rb efflux and possibilities of its influence.人体红细胞离子转运控制的研究。I. 86Rb的被动外流及其影响因素
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Activation by N-ethylmaleimide of a latent K+-Cl- flux in human red blood cells.N-乙基马来酰亚胺对人红细胞中潜在钾氯通量的激活作用。
Am J Physiol. 1984 May;246(5 Pt 1):C385-90. doi: 10.1152/ajpcell.1984.246.5.C385.

引用本文的文献

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Regulation of K-Cl cotransport: from function to genes.钾氯共转运体的调节:从功能到基因
J Membr Biol. 2004 Oct 1;201(3):109-37. doi: 10.1007/s00232-004-0695-6.
2
Cytoplasmic pH and human erythrocyte shape.细胞质pH值与人类红细胞形态
Biophys J. 1997 Mar;72(3):1234-46. doi: 10.1016/S0006-3495(97)78770-8.