大鼠急性肾移植排斥反应中白细胞介素-17 的变化及意义。
Changes and Significance of IL-17 in Acute Renal Allograft Rejection in Rats.
机构信息
Inner Mongolia Medical University, Huhhot, China.
Inner Mongolia Autonomous Region Tongliao City Hospital, Tongliao, China.
出版信息
Transplant Proc. 2022 Sep;54(7):2021-2024. doi: 10.1016/j.transproceed.2022.05.019. Epub 2022 Aug 4.
BACKGROUND
To investigate the changes and significance of interleukin-17 (IL-17) in acute rejection following rat kidney transplantation.
METHODS
Using inbred Sprague Dawley rats as donors and Wistar rats as recipients, an acute rejection model of kidney transplantation was established to evaluate the effects of IL-17. Reverse transcription polymerase chain reaction and immunohistochemistry were used to detect IL-17.
RESULTS
Compared with those in the normal control group, the rats in the allogeneic transplantation (ATX) group had different degrees of acute rejection 3, 5, and 7 days after operation, and the expression of IL-17 mRNA in the transplanted kidney was significantly increased (P < .05). In the ATX group, acute rejection was observed 7 days after operation, and the integrated optical density (IOD) value of IL-17 was significantly increased (P < .05). Compared with the normal control group, acute rejection occurred in varying degrees at 3, 5, and 7 days after operation in the ATX group, and the IOD value of IL-17 significantly increased (P < .05).
CONCLUSIONS
IL-17 expression is increased in acute rejection after renal transplantation in rats. Other surgical factors in addition to acute rejection had no effect on IL-17 expression in rat kidney transplants. The immunosuppressant cyclosporin A was used to prevent the expression of IL-17 in rats with acute rejection.
背景
探讨白细胞介素-17（IL-17）在大鼠肾移植后急性排斥反应中的变化及意义。
方法
以内生性 Sprague Dawley 大鼠作为供体，Wistar 大鼠作为受体，建立肾移植急性排斥反应模型，评价 IL-17 的作用。采用逆转录聚合酶链反应和免疫组织化学法检测 IL-17。
结果
与正常对照组相比，同种异体移植（ATX）组大鼠术后 3、5、7 天出现不同程度的急性排斥反应，移植肾 IL-17mRNA 表达明显增加（P<0.05）。在 ATX 组中，术后 7 天观察到急性排斥反应，IL-17 的积分光密度（IOD）值明显增加（P<0.05）。与正常对照组相比，ATX 组大鼠术后 3、5、7 天均出现不同程度的急性排斥反应，IL-17 的 IOD 值明显增加（P<0.05）。
结论
大鼠肾移植后急性排斥反应时 IL-17 表达增加。除急性排斥反应外，其他手术因素对大鼠移植肾 IL-17 的表达没有影响。免疫抑制剂环孢素 A 可预防急性排斥反应大鼠 IL-17 的表达。
Zotero 插件