Chi Zixin, Wu Xi, Zhang Qiqi, Zhai Fengyang, Xu Zesheng, Zhang Dandan, Chen Qing
School of Pharmacy, School of Public Health, Shenyang Medical College, Shenyang, P. R. China.
College of Chemistry, Liaoning University, Shenyang, P. R. China.
J Sep Sci. 2022 Oct;45(19):3754-3762. doi: 10.1002/jssc.202200357. Epub 2022 Aug 11.
Titanium-based metal-organic framework MIL-125(Ti) was synthesized by the hydrothermal method of terephthalic acid and tetra butyl titanate in N-N,dimethylformamide, and methanol. MIL-125(Ti) was characterized by Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, nitrogen adsorption-desorption, energy-dispersive X-ray spectroscopy, zeta potential, scanning electron microscope, and transmission electron microscopy. The results showed MIL-125(Ti) could be used as a potential adsorbent for protein separation and purification due to the high specific surface area, high stability, and strong hydrophobicity. As a result, MIL-125(Ti) had adsorption selectivity for immunoglobulin G, which was due to the hydrogen bond between MIL-125(Ti) and protein. At pH 8.0, the maximum adsorption efficiency of 0.25 mg MIL-125(Ti) for 300 μL 100 μg/mL immunoglobulin G was 98.3%, and its maximum adsorption capacity was 232.56 mg/g. The elution efficiency of immunoglobulin G was 92.4% by 0.1% sodium dodecyl sulfate. sodium dodecyl sulfate-polyacrylamide gel electrophoresis result demonstrated the successful isolation of highly purified immunoglobulin G from the human serum. Therefore, a new method of separation and purification of immunoglobulin G in human serum using titanium-based metal-organic framework MIL-125(Ti) as a solid-phase adsorbent was established, which broadened the application scope of metal-organic frameworks.
采用对苯二甲酸和钛酸四丁酯在N，N -二甲基甲酰胺和甲醇中进行水热法合成了钛基金属有机框架材料MIL - 125(Ti)。通过傅里叶变换红外光谱、X射线衍射、热重分析、氮气吸附-脱附、能量色散X射线光谱、zeta电位、扫描电子显微镜和透射电子显微镜对MIL - 125(Ti)进行了表征。结果表明，由于具有高比表面积、高稳定性和强疏水性，MIL - 125(Ti)可作为蛋白质分离纯化的潜在吸附剂。因此，MIL - 125(Ti)对免疫球蛋白G具有吸附选择性，这是由于MIL - 125(Ti)与蛋白质之间存在氢键。在pH 8.0时，0.25 mg MIL - 125(Ti)对300 μL 100 μg/mL免疫球蛋白G的最大吸附效率为98.3%，其最大吸附容量为232.56 mg/g。用0.1%十二烷基硫酸钠洗脱免疫球蛋白G的效率为92.4%。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳结果表明成功从人血清中分离出了高纯度的免疫球蛋白G。因此，建立了一种以钛基金属有机框架MIL - 125(Ti)为固相吸附剂分离纯化人血清中免疫球蛋白G的新方法，拓宽了金属有机框架材料的应用范围。
