A new ribonuclease from cobra venom (Naja naja) showing specificity towards cytidylic acid.
作者信息
Mahalakshmi Y V, Pandit M W
出版信息
Biochem Biophys Res Commun. 1987 Jun 15;145(2):740-8. doi: 10.1016/0006-291x(87)91027-8.
PMID:3593367
Abstract
A ribonuclease was isolated and purified to homogeneity from cobra venom. The enzyme was found to be homogeneous on SDS and non-SDS polyacrylamide gel electrophoreses, and high performance liquid chromatography. The purified enzyme hydrolysed poly(rC), but not poly(rA), poly(rU), poly(rG), poly(rI), or poly(rI).poly(rC). The presence of magnesium was found to be an essential requirement for the nucleolytic activity of this enzyme.
摘要
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