Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University, Guangdong, China; Guangdong Province Translational Forensic Medicine Engineering Technology Research Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangdong, China.
Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, China.
Forensic Sci Int. 2022 Oct;339:111409. doi: 10.1016/j.forsciint.2022.111409. Epub 2022 Jul 22.
Y-chromosomal short tandem repeats (Y-STRs) with high mutation rates will increase the potential to distinguish related males and improve discrimination capacity. The newly developed Y32 kit is a six-dye multiplex amplification kit that contains 21 rapidly mutating Y-STR loci (DYF387S1a/b, DYF399S1a/b/c, DYF403S1a1/a2/a3/b, DYF404S1a/b, DYS449, DYS518, DYS526a/b, DYS547, DYS570, DYS576, DYS612, DYS626, and DYS627), 8 fast mutating Y-STR loci (DYS458, DYS464a/b/c/d, DYS516, DYS534, and DYS713), and 3 moderately mutating Y-STR loci (Y-GATA-A10, DYS630, and DYS446). To verify the efficiency and accuracy of the Y32 kit, PCR reaction conditions, sensitivity, mixture, concordance, inhibition, species specificity, case samples, reproducibility, sizing precision, stutter, and population study were studied according to the SWGDAM developmental validation guidelines. The results showed that the Y32 kit is efficient, accurate, reliable, and highly informative for forensic applications.
Y 染色体短串联重复序列（Y-STRs）具有较高的突变率，这将增加区分相关男性的潜力并提高鉴别能力。新开发的 Y32 试剂盒是一种六染料多重扩增试剂盒，包含 21 个快速突变的 Y-STR 基因座（DYF387S1a/b、DYF399S1a/b/c、DYF403S1a1/a2/a3/b、DYF404S1a/b、DYS449、DYS518、DYS526a/b、DYS547、DYS570、DYS576、DYS612、DYS626 和 DYS627）、8 个快速突变的 Y-STR 基因座（DYS458、DYS464a/b/c/d、DYS516、DYS534 和 DYS713）和 3 个中度突变的 Y-STR 基因座（Y-GATA-A10、DYS630 和 DYS446）。为了验证 Y32 试剂盒的效率和准确性，根据 SWGDAM 开发验证指南研究了 PCR 反应条件、灵敏度、混合物、一致性、抑制、种属特异性、案例样本、可重复性、大小精度、微卫星不稳定性和群体研究。结果表明，Y32 试剂盒对于法医应用是高效、准确、可靠和高度信息丰富的。
