Zhang Hongliang, Zhang Ruihua, Wang Fengxue, Li Guimei, Wen Yongjun, Shan Hu
Ministry of Agriculture Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Diseases, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, Inner Mongolia, China; Shandong Collaborative Innovation Center for Development of Veterinary Pharmaceuticals, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, Shandong, China.
Key Laboratory of Preventive Veterinary Medicine, Department of Veterinary Medicine, Animal Science College, HeBei North University, Zhangjiakou, 075131, Hebei, China.
Microb Pathog. 2022 Sep;170:105698. doi: 10.1016/j.micpath.2022.105698. Epub 2022 Aug 5.
Pseudorabies virus (PRV) is the causative agent of Aujeszky's disease and is communicable across species. In particular, the emergence of PRV variants in 2011 have resulted in serious economic losses to the Chinese pig industry. In this study, we used tandem mass tag (TMT) quantitative protein analysis to identify differentially expressed proteins between the PRV variant strain SD-2017 and the vaccine strain Bartha-K/61 in the swine kidney cell line PK15. Overall, we identified 4690 proteins for SD-2017 infection compared with the mock-infected control cells. We found 162 differentially expressed cellular proteins including 41 up- and 121 down-regulated proteins. SD-2017-infected PK15 cells differential proteins were primarily related to gap junctions, the phagosome, antigen processing and presentation, cell adhesion molecules and peroxisome pathways. Compared to Bartha-K/61-infected PK15 cells, SD-2017-infected cells displayed differentially expressed proteins involved in tryptophan metabolism, mitophagy and Notch signaling. Western blot analysis of MARK2, TSR1 and TMED1 three representative proteins validated the reliability of the TMT data. This study is an initial at-tempt to compare the proteomes of PK15 cells infected by a PRV variant and a vaccine strain using TMT technology to provide new insights into the mechanisms of PRV pathogenesis and immune evasion.
伪狂犬病病毒（PRV）是奥耶斯基氏病的病原体，可在物种间传播。特别是2011年PRV变异株的出现给中国养猪业造成了严重的经济损失。在本研究中，我们使用串联质谱标签（TMT）定量蛋白质分析来鉴定猪肾细胞系PK15中PRV变异株SD - 2017和疫苗株Bartha-K/61之间的差异表达蛋白质。总体而言，与 mock 感染的对照细胞相比，我们鉴定出了4690种SD - 2017感染相关的蛋白质。我们发现了162种差异表达的细胞蛋白质，包括41种上调和121种下调的蛋白质。SD - 2017感染的PK15细胞差异蛋白质主要与间隙连接、吞噬体、抗原加工和呈递、细胞粘附分子以及过氧化物酶体途径有关。与Bartha-K/61感染的PK15细胞相比，SD - 2017感染的细胞显示出参与色氨酸代谢、线粒体自噬和Notch信号传导的差异表达蛋白质。对MARK2、TSR1和TMED1三种代表性蛋白质的蛋白质免疫印迹分析验证了TMT数据的可靠性。本研究首次尝试使用TMT技术比较PRV变异株和疫苗株感染的PK15细胞的蛋白质组，为PRV发病机制和免疫逃逸机制提供新的见解。
