Laboratory of Immunology and Molecular Biology, Institute of Health Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon, s/n-Canela, Salvador, Brazil.
Curr Microbiol. 2022 Aug 8;79(9):283. doi: 10.1007/s00284-022-02974-7.
Corynebacterium pseudotuberculosis is a bacillus that causes caseous lymphadenitis in small ruminants, leading to great losses to rural producers; thus, an efficient diagnosis is necessary for using disease control measures. This study aimed to evaluate the antigenic potential of four C. pseudotuberculosis recombinant proteins (rSodC, rPknG, rNanH, and rSpaC) against sera of goat and sheep experimentally infected with one of three different C. pseudotuberculosis strains. Goats were infected with CAP76 or CAP21 strain (n = 10), sheep with VD57 strain (n = 6), and a group of not-infected animals (goats and sheep) were kept as a healthy control (healthy n = 12). Sera were collected at 0, 14, 60, 90, 180, or 190 days after inoculation for antigenicity testing using Western blotting and enzyme-linked immunosorbent assay (ELISA) techniques. Cross-reactivity tests with recombinant proteins were performed in goat serum experimentally vaccinated with Nocardia sp. or Rhodococcus equi bacterin. The rSodC protein showed discriminatory antigenic reactivity with a statistically significant difference against three different C. pseudotuberculosis strains evaluated in goats and sheep samples, while rPknG showed statistical significance only against two C. pseudotuberculosis strains evaluated in goats. rSodC was proved to be a strong candidate as a tool for diagnosis of C. pseudotuberculosis infection, once it was able to recognize antibodies against all strains evaluated in goats and sheep.
类白喉棒状杆菌是一种杆菌，可引起小反刍动物干酪性淋巴结炎，给农村生产者造成巨大损失；因此，需要进行有效的诊断，以采取疾病控制措施。本研究旨在评估四种类白喉棒状杆菌重组蛋白（rSodC、rPknG、rNanH 和 rSpaC）对实验感染三种不同类白喉棒状杆菌菌株之一的山羊和绵羊血清的抗原性。山羊感染 CAP76 或 CAP21 菌株（n=10），绵羊感染 VD57 菌株（n=6），一组未感染动物（山羊和绵羊）作为健康对照组（健康 n=12）。在接种后 0、14、60、90、180 或 190 天采集血清，用于 Western blot 和酶联免疫吸附试验（ELISA）技术的抗原性检测。使用重组蛋白进行交叉反应试验，在山羊血清中用奴卡氏菌或马红球菌菌苗进行实验性接种。rSodC 蛋白与三种不同的类白喉棒状杆菌菌株在山羊和绵羊样本中的反应具有明显的鉴别性抗原反应，而 rPknG 蛋白仅与山羊中评估的两种类白喉棒状杆菌菌株具有统计学意义。rSodC 被证明是一种很有前途的工具，可用于诊断类白喉棒状杆菌感染，因为它能够识别所有在山羊和绵羊中评估的菌株的抗体。
