Fritze J, Fröhlich U, Beckmann H
Biol Chem Hoppe Seyler. 1987 Mar;368(3):295-8. doi: 10.1515/bchm3.1987.368.1.295.
Genetic variants of human plasma butyrylcholinesterase have been characterized and are highly relevant to anesthesiology. They might also represent potential genetic markers for neuropsychiatric disorders. Two-dimensional electrophoresis with isoelectrofocusing in the first and polyacrylamide gel electrophoresis in the second dimension has proved to be a powerful tool in search for genetic variants. Butyrylcholinesterase is an oligomeric enzyme with considerable charge heterogeneity. Conventional two-dimensional electrophoresis proved unsuitable for this enzyme possibly due to its tendency to aggregate by hydrophobic interactions. The inversion of the sequence applying polyacrylamide gel electrophoresis in the first and isoelectric focusing in the second dimension circumvented this problem.
人类血浆丁酰胆碱酯酶的基因变体已得到表征，且与麻醉学高度相关。它们也可能代表神经精神疾病的潜在遗传标记。先进行等电聚焦、再进行聚丙烯酰胺凝胶电泳的二维电泳已被证明是寻找基因变体的有力工具。丁酰胆碱酯酶是一种具有相当电荷异质性的寡聚酶。传统的二维电泳可能因其倾向于通过疏水相互作用聚集而被证明不适用于这种酶。将顺序颠倒，先进行聚丙烯酰胺凝胶电泳、再进行等电聚焦，解决了这个问题。
