Siminovitch D J, Wong P T, Mantsch H H
Biochim Biophys Acta. 1987 Jun 12;900(1):163-7. doi: 10.1016/0005-2736(87)90289-6.
High-pressure infrared spectroscopy is used to compare the barotropic behaviour of various interdigitated lipid bilayer systems (1,2-di-O-hexadecyl-sn-glycero-3-phosphocholine, 1,3-dipalmitoyl-sn-glycero-2-phosphocholine and 1-palmitoyl-sn-glycero-3-phosphocholine) with non-interdigitated bilayers of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine. In the pressure range between 0 and 20 kbar, we have monitored the pressure dependence of the relative peak height intensity ratio of the chain methylene scissoring band, delta CH2, and its correlation field component band, delta'CH2. We demonstrate that this parameter, in conjunction with a visual inspection of the pressure-induced correlation field splittings of the methylene scissoring and rocking mode bands, can provide reliable indications of chain interdigitation.
高压红外光谱用于比较各种交错排列的脂质双层系统（1,2 - 二 - O - 十六烷基 - sn - 甘油 - 3 - 磷酸胆碱、1,3 - 二棕榈酰 - sn - 甘油 - 2 - 磷酸胆碱和1 - 棕榈酰 - sn - 甘油 - 3 - 磷酸胆碱）与1,2 - 二棕榈酰 - sn - 甘油 - 3 - 磷酸胆碱的非交错双层的正压行为。在0至20千巴的压力范围内，我们监测了链亚甲基剪式振动带δCH₂及其相关场分量带δ'CH₂的相对峰高强度比的压力依赖性。我们证明，该参数与对亚甲基剪式振动和摇摆模式带的压力诱导相关场分裂的目视检查相结合，可以提供链交错排列的可靠指示。
