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从嫁接到番茄上的杂交马铃薯植株高效获取后代种子的方法。

Procedure for the efficient acquisition of progeny seeds from crossed potato plants grafted onto tomato.

作者信息

Takeuchi Ami, Akatsu Yuna, Asahi Takahiro, Okubo Yukino, Ohnuma Mariko, Teramura Hiroshi, Tamura Koji, Shimada Hiroaki

机构信息

Department of Biological Science and Technology, Tokyo University of Science, Katsushika, Tokyo 125-8585, Japan.

出版信息

Plant Biotechnol (Tokyo). 2022 Jun 25;39(2):195-197. doi: 10.5511/plantbiotechnology.21.1119a.

DOI:10.5511/plantbiotechnology.21.1119a
PMID:35937528
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9300436/
Abstract

Potato, L. is an important crop. However, it is difficult to breed potato cultivars by applying conventional crossing methods because potato has a tetraploid genome and is vegetatively propagated. Flower formation and tuber development occur simultaneously. Many potato cultivars hardly produce any fruits after crossing and fail to produce seeds. We report an improved procedure for obtaining progeny seeds by grafting potatoes onto tomatoes. The rate of fruit formation was more than 19% when the grafted potatoes were used for the crossing experiments, whereas crossing using the ungrafted plants showed a rate of 1.1%. This result suggests that our procedure results in the easy acquisition of null-segregant progenies by crossing mutant lines. It is also expected to improve conventional potato breeding.

摘要

马铃薯(Solanum tuberosum L.)是一种重要的作物。然而,由于马铃薯具有四倍体基因组且通过无性繁殖,应用传统杂交方法培育马铃薯品种很困难。花的形成和块茎的发育同时发生。许多马铃薯品种杂交后几乎不产生任何果实,也无法产生种子。我们报告了一种通过将马铃薯嫁接到番茄上获得后代种子的改进方法。当将嫁接的马铃薯用于杂交实验时,坐果率超过19%,而使用未嫁接植株进行杂交的坐果率为1.1%。这一结果表明,我们的方法能够通过杂交突变系轻松获得零分离后代。它还有望改进传统的马铃薯育种。

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引用本文的文献

1
Pollen transcriptomic analysis provided insights into understanding the molecular mechanisms underlying grafting-induced improvement in potato fertility.花粉转录组分析为理解嫁接诱导马铃薯育性提高的分子机制提供了见解。
Front Plant Sci. 2024 Mar 28;15:1338106. doi: 10.3389/fpls.2024.1338106. eCollection 2024.
2
Genome-wide characterization of gene family in potato () and expression analyses under salt and hormone stress.马铃薯(Solanum tuberosum)基因家族的全基因组特征分析及盐胁迫和激素胁迫下的表达分析
Front Plant Sci. 2023 Jun 30;14:1201730. doi: 10.3389/fpls.2023.1201730. eCollection 2023.

本文引用的文献

1
Creation of a potato mutant lacking the starch branching enzyme gene that was generated by genome editing using the CRISPR/dMac3-Cas9 system.利用CRISPR/dMac3-Cas9系统通过基因组编辑创建的缺乏淀粉分支酶基因的马铃薯突变体。
Plant Biotechnol (Tokyo). 2021 Sep 25;38(3):345-353. doi: 10.5511/plantbiotechnology.21.0727a.
2
Genome Editing for Crop Improvement - Applications in Clonally Propagated Polyploids With a Focus on Potato ( L.).用于作物改良的基因组编辑——以马铃薯(茄属)为重点的无性繁殖多倍体中的应用
Front Plant Sci. 2018 Nov 13;9:1607. doi: 10.3389/fpls.2018.01607. eCollection 2018.
3
Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato.利用翻译增强子 dMac3 和多个向导 RNA 在马铃薯中建立具有更高诱变频率的改良 CRISPR/Cas9 系统。
Sci Rep. 2018 Sep 13;8(1):13753. doi: 10.1038/s41598-018-32049-2.