Timofeeva N G, Martynova M A, Pokrovskiĭ S N, Adamova I Iu, Prokazova N V
Biokhimiia. 1987 Apr;52(4):650-4.
Gangliosides have been shown to modulate the receptor-mediated endocytosis of low density lipoproteins (LDL). The direct interaction of LDL with various gangliosides has been studied. Binding of gangliosides to LDL immobilized on CNBr-Sepharose (LDL-Sepharose) and the influence of gangliosides on the fluorescence of LDL containing anthrylvinyl-labeled sphingomyelin were investigated. The binding of 3H-gangliosides to LDL-Sepharose, as well as fluorescence polarization of LDL were found to depend on the structure and concentration of the gangliosides in a specific saturable manner. The data obtained indicate that gangliosides interact with apolipoprotein B via specific binding sites.
神经节苷脂已被证明可调节低密度脂蛋白（LDL）的受体介导内吞作用。人们对LDL与各种神经节苷脂的直接相互作用进行了研究。研究了神经节苷脂与固定在溴化氰-琼脂糖（LDL-琼脂糖）上的LDL的结合，以及神经节苷脂对含有蒽乙烯基标记鞘磷脂的LDL荧光的影响。发现3H-神经节苷脂与LDL-琼脂糖的结合以及LDL的荧光偏振以特定的饱和方式取决于神经节苷脂的结构和浓度。所获得的数据表明，神经节苷脂通过特定结合位点与载脂蛋白B相互作用。
