Liu Shoude, Liu Huamei, Zhou Li, Cheng Zhiguo, Wan Jun, Pan Yu, Xu Guang, Huang Fang, Wang Meng, Xiong Yuanyuan, Hu Guoyuan
Key Laboratory for Green Chemical Process of Ministry of Education, School of Environmental Ecology and Bioengineering, Wuhan Institute of Technology, Wuhan, China.
Department of Research and Development, Wuhan Kernel Bio-tech Co., Ltd., Wuhan, China.
J Appl Microbiol. 2022 Nov;133(5):2954-2965. doi: 10.1111/jam.15750. Epub 2022 Aug 8.
We aimed to enhance the antibacterial and growth-promoting effects of Paenibacillus polymyxa by improving the yield of spores, lipopeptides and indole-3-acetic acid (IAA) in the fermentation process.
Through medium optimization by the response surface method and feeding fermentation, the number of spores reached 2.37 × 10 cfu ml with an increase of 38%, the content of lipopeptides reached 60.8 mg L with an increase of 89%, and the content of IAA reached 24.3 mg L with an increase of 176%, respectively, comparing with the original (un-optimized) culture conditions. The fermentation culture of P. polymyxa from the optimized medium and feeding fermentation resulted in higher colonization of P. polymyxa in soils than that from the original culture during the 49 days for testing. Comparing with the supernatant of the original culture, the supernatant of the P. polymyxa culture from the optimized medium and feeding fermentation showed enhanced antibacterial effects and plant growth-promoting effects. The enhanced antibacterial effect was shown as the increase of the inhibition zone by 59%, 45% and 26% against Ralstonia solanacearum, Erwinia carotovora and Xanthomonas campestris. The enhanced growth-promoting effects on tomato and strawberry plants were the increase of plant height by 47% and 5%, root length by 23% and 15% and root weight by 65% and 110%.
The combination of medium optimization and feeding fermentation effectively improved the yield of spores, lipopeptides and IAA. Lipopeptides and IAA lead to enhanced antibacterial and plant growth-promoting effects of the P. polymyxa product.
The optimized fermentation method significantly improved the yield of spores, lipopeptides and IAA, thus providing theoretical and technical support for enhancing the antibacterial and growth-promoting effects of P. polymyxa products in agriculture.
我们旨在通过提高发酵过程中多粘芽孢杆菌的孢子、脂肽和吲哚 - 3 - 乙酸（IAA）产量，增强其抗菌和促生长作用。
通过响应面法进行培养基优化和补料发酵，与原始（未优化）培养条件相比，孢子数达到2.37×10⁹ cfu/ml，增加了38%；脂肽含量达到60.8 mg/L，增加了89%；IAA含量达到24.3 mg/L，增加了176%。在49天的测试期间，来自优化培养基和补料发酵的多粘芽孢杆菌发酵培养物在土壤中的定殖率高于原始培养物。与原始培养物的上清液相比，来自优化培养基和补料发酵的多粘芽孢杆菌培养物的上清液显示出增强的抗菌作用和促进植物生长的作用。增强的抗菌作用表现为对青枯雷尔氏菌、胡萝卜软腐欧文氏菌和野油菜黄单胞菌的抑菌圈分别增加了59%、45%和26%。对番茄和草莓植株增强的促生长作用表现为株高分别增加了47%和5%，根长分别增加了23%和15%，根重分别增加了65%和110%。
培养基优化和补料发酵相结合有效提高了孢子、脂肽和IAA的产量。脂肽和IAA导致多粘芽孢杆菌产品的抗菌和促进植物生长作用增强。
优化的发酵方法显著提高了孢子、脂肽和IAA的产量，从而为增强多粘芽孢杆菌产品在农业中的抗菌和促生长作用提供了理论和技术支持。
