Termination of pregnancy in mice with antiserum to chicken riboflavin-carrier protein.

The importance of riboflavin-carrier protein (RCP) in the maintenance of pregnancy in mice has been studied. Selective passive immunoneutralization of the maternal RCP resulted in fetal death and resorption. Six hours after chicken RCP antiserum treatment, the following observations were made: there was profuse vaginal bleeding in all the animals, a 60% reduction in embryonic ornithine decarboxylase (ODC) activity, a 70% reduction in the maternal progesterone levels, and a 50% reduction in the 14C-riboflavin uptake by the embryo. The above observations are indicative of fetal distress and resorption. By 24 h after treatment, there was 100% resorption of fetuses and the mouse progesterone levels dropped to 20% of untreated or normal rabbit serum (NRS)-treated values. Cytological studies of the fetal liver revealed the classical signs of cellular degeneration in hepatocytes as well as hematopoietic cells. The effect was apparent as early as 1 h after antiserum administration. The erythroid aplasia supports the biochemical evidence that fetal demise is due to preferential riboflavin deficiency of the fetus.