McEvoy R C, Hegre O D
Diabetes. 1978 Oct;27(10):988-95. doi: 10.2337/diab.27.10.988.
Eight 18-days-postcoitum fetal pancreases were transplanted to isogenic alloxan-diabetic male rats. Some recipients were treated with insulin for seven days immediately after transplantation. Eight animals in both the insulin-treated group and control group were killed 15days after transplantation for morphologic and hormonal studies of the transplanted tissue. Using the morphometric technique of linear scanning, the insulin, glucagon, and somatostatin immunocytochemically positive, cell masses of the fetal pancreatic implants were quantitated. The beta cell mass of the implants from the control animals increased roughly eightfold from the time of transplant; insulin treatment resulted in a further two- to threefold increase. The insulin content of the implants increased more than did the beta cell mass, resulting in the fivefold increase in insulin per beta cell. The alpha cell and delta cell masses did not change during the transplant site, the mass of functional beta cells, and the cell-to-cell content of the implanted tissue. These results are discussed in relation to previous quantitative studies of pancreatic islet cell growth. The relationships of the transplant site, the mass of functional beta cell, and the cell-to-cell interaction within the islet to the maintenance of glucose homeostasis are also discussed.
将8个妊娠18天的胎儿胰腺移植到同基因的四氧嘧啶糖尿病雄性大鼠体内。部分受体在移植后立即接受了7天的胰岛素治疗。胰岛素治疗组和对照组各有8只动物在移植后15天处死，以便对移植组织进行形态学和激素研究。采用线性扫描形态计量技术，对胎儿胰腺移植物中胰岛素、胰高血糖素和生长抑素免疫细胞化学阳性的细胞团进行定量分析。对照组动物移植物的β细胞团从移植时起大致增加了8倍；胰岛素治疗使其进一步增加了2至3倍。移植物的胰岛素含量比β细胞团增加得更多，导致每个β细胞的胰岛素含量增加了5倍。α细胞团和δ细胞团在移植过程中没有变化，研究了移植部位、功能性β细胞团以及植入组织的细胞间含量。结合先前对胰岛细胞生长的定量研究对这些结果进行了讨论。还讨论了移植部位、功能性β细胞团以及胰岛内细胞间相互作用与维持葡萄糖稳态的关系。
