双极管微秒脉冲大气压等离子体射流阵列制备的等离子体激活介质诱导人宫颈癌细胞线粒体介导的细胞凋亡。
Plasma activated medium prepared by a bipolar microsecond-pulsed atmospheric pressure plasma jet array induces mitochondria-mediated apoptosis in human cervical cancer cells.
机构信息
Department of Biological Sciences, Dong-A University, Busan, Korea.
Department of Physics, Dong-A University, Busan, Korea.
出版信息
PLoS One. 2022 Aug 8;17(8):e0272805. doi: 10.1371/journal.pone.0272805. eCollection 2022.
Plasma activated medium (PAM) was prepared by a bipolar microsecond-pulsed atmospheric pressure plasma jet (APPJ) array source and was utilized for cancer cell treatment. APPJ array-produced plasma were characterized. APPJ array treatment of three different solutions (deionized water (DW), HBSS (serum-free Hanks' balanced salt solution), and DMEM (Dulbecco's Modified Eagle Medium) + 10% FBS (fetal bovine serum)) were performed to induce the changes in the concentration of reactive oxygen and nitrogen species (RONS) as functions of the operating parameters. Human cervical cancer cells (HeLa) injected with plasma-treated media were investigated for changes in cell viability using MTT assay. It was observed that PAM-induced ROS can regulate the protein expression associated with mitochondria, and PAM causes apoptosis through Cyto C/JNK/p38 signaling on human cervical cancer cells.
等离子体激活介质（PAM）由双极微秒脉冲大气压等离子体射流（APPJ）阵列源制备，并用于癌细胞处理。对 APPJ 阵列产生的等离子体进行了特性分析。对 APPJ 阵列处理三种不同溶液（去离子水（DW）、HBSS（无血清 Hank's 平衡盐溶液）和 DMEM（Dulbecco's Modified Eagle Medium）+10% FBS（胎牛血清））的情况进行了研究，以作为操作参数的函数，来诱导活性氧和氮物种（RONS）浓度的变化。用 MTT 测定法研究了注入经等离子体处理的培养基的人宫颈癌（HeLa）细胞的细胞活力变化。结果表明，PAM 诱导的 ROS 可以调节与线粒体相关的蛋白质表达，并且 PAM 通过 Cyto C/JNK/p38 信号通路导致人宫颈癌细胞凋亡。
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