Zhang Yuanyuan, Kang Liru, Gao Jing, Puri Krishna D, Jia Ruifang, Zhang Zhiwei, Zhang Jian, Zhao Jun
College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010018, China.
Key Laboratory of Biohazard Monitoring and Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of CAAS, Hohhot 010010, China.
Plant Dis. 2023 Mar;107(3):750-757. doi: 10.1094/PDIS-05-22-1029-RE. Epub 2023 Mar 20.
A green fluorescent protein (GFP)-tagged isolate of was used to study its colonization in potato plants and tubers. Three-week-old potato plants of the highly susceptible cultivar 'Shepody' were inoculated with a conidial suspension of a GFP-tagged isolate of using a wound inoculation method. Colonization was studied using confocal microscopy combined with tissue sections. Conidia germinated and hyphae grew along the root hairs, elongation zones, and root caps between 24 and 96 h postinoculation (HPI). At 7 days postinoculation (DPI), the pathogen advanced to cortical tissues and grew into the root vascular bundles. At 8 weeks postinoculation (WPI), the stem epidermal cells, cortical tissues, vascular elements, and petioles were fully colonized by the mycelium of . At 11 WPI, the pathogen was detected in the stolon and progeny tubers, as confirmed by both GFP signals in tissues and reisolation of the pathogen on the semiselective NP-10 medium. Progeny potato tubers were harvested from the inoculated potato plants, and the GFP-signal was observed in the epidermal cells and vascular elements of sprouting buds that emerged from the harvested tubers. The infection rate of progeny tubers detected on semiselective NP-10 medium ranged from 34.55 to 55.56%, with an average of 45.31%. In conclusion, we report, for the first time, the entire progression of colonization by in potato plant tissues, progeny tubers, as well as of the sprouting buds that emerged from progeny tubers.
使用绿色荧光蛋白（GFP）标记的[某种病原体名称未给出]分离株来研究其在马铃薯植株和块茎中的定殖情况。采用伤口接种法，用GFP标记的[某种病原体名称未给出]分离株的分生孢子悬浮液接种三周龄的高度易感品种‘Shepody’马铃薯植株。使用共聚焦显微镜结合组织切片研究定殖情况。接种后24至96小时（HPI），分生孢子萌发，菌丝沿着根毛、伸长区和根冠生长。接种后7天（DPI），病原体侵入皮层组织并生长到根维管束中。接种后8周（WPI），茎表皮细胞、皮层组织、维管束和叶柄被[某种病原体名称未给出]的菌丝完全定殖。接种后11周（WPI），在匍匐茎和后代块茎中检测到病原体，组织中的GFP信号以及病原体在半选择性NP - 10培养基上的再分离均证实了这一点。从接种的马铃薯植株上收获后代马铃薯块茎，在收获块茎上萌发的芽的表皮细胞和维管束中观察到GFP信号。在半选择性NP - 10培养基上检测到的后代块茎感染率在34.55%至55.56%之间,平均为45.31%。总之，我们首次报道了[某种病原体名称未给出]在马铃薯植株组织、后代块茎以及后代块茎上萌发的芽中的整个定殖过程。
