中华乌塘鳢（Bostrychus sinensis）和云纹石斑鱼（Epinephelus moara）维生素 B12 吸收相关基因的鉴定与分析
Molecular characterization and expression analysis of interleukin-15 (IL-15) genes in orange-spotted grouper (Epinephelus coioides) in response to Vibrio harveyi challenge.
机构信息
Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518120, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, 51820, China; College of Fishery, Guangdong Ocean University, Zhanjiang, 524025, China; Guangdong Provincial Key Laboratory of Aquaic Animal Disease Control and Healthy Culture, Zhanjiang, 524025, China.
College of Fishery, Guangdong Ocean University, Zhanjiang, 524025, China; Guangdong Provincial Key Laboratory of Aquaic Animal Disease Control and Healthy Culture, Zhanjiang, 524025, China.
出版信息
Fish Shellfish Immunol. 2022 Sep;128:327-334. doi: 10.1016/j.fsi.2022.08.003. Epub 2022 Aug 5.
As a member of the γc family, interleukin 15 plays an important function in the immune response. In this study, we cloned an IL15 from Epinephelus coioides (named Ec-IL15). The open reading frame of Ec-IL15 is 528 bp, encoding 175 amino acids. Sequence alignment analysis showed that EcIL-15 has a conserved Pfam: IL15 domain and four cysteine residues. Subcellular localization studies have shown that Ec-IL15 is distributed in whole cells. In healthy groupers, Ec-IL15 was expressed in all 11 tissues tested and the highest in liver. After ConA, PHA, LPS and poly I:C stimulation, Ec-IL15 expression of HKLs was significantly upregulated. After V. harveyi infection, the expression of Ec-IL15 in 9 tissues was significantly upregulated and peaked within 48 h. In addition, recombinant Ec-IL15 protein can not only stimulate HKLs proliferation and cytokine expression, but also has the potential as an immune enhancer.
作为 γc 家族的一员，白细胞介素 15 在免疫反应中发挥着重要作用。本研究从卵形鲳鲹（Epinephelus coioides）克隆了白细胞介素 15（命名为 Ec-IL15）。Ec-IL15 的开放阅读框为 528bp，编码 175 个氨基酸。序列比对分析表明，EcIL-15 具有保守的 Pfam：IL15 结构域和四个半胱氨酸残基。亚细胞定位研究表明，Ec-IL15 分布在整个细胞中。在健康的石斑鱼中，Ec-IL15 在检测的 11 种组织中均有表达，在肝脏中表达量最高。在 ConA、PHA、LPS 和 poly I:C 刺激后，HKLs 的 Ec-IL15 表达显著上调。在哈氏弧菌感染后，9 种组织中 Ec-IL15 的表达显著上调，在 48h 时达到峰值。此外，重组 Ec-IL15 蛋白不仅能刺激 HKLs 的增殖和细胞因子表达，而且具有作为免疫增强剂的潜力。
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