Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz - FIOCRUZ, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, RJ, Brazil.
Programa de Pós-graduação em Biologia Parasitária, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil.
J Helminthol. 2022 Aug 9;96:e60. doi: 10.1017/S0022149X22000244.
The parasite biodiversity of mouse opossums in Brazil remains incompletely explored. We describe a new species of (Ascaridida: Subuluroidea) from the large intestine of the white-bellied woolly mouse opossum, , based on the results of light and scanning electron microscopy (SEM). We also partially sequenced the mitochondrial cytochrome c oxidase I (MT-CO1) gene of the new species, using molecular phylogenetic analyses to determine its relationships within the Subuluroidea superfamily. As molecular data on subuluroid species are extremely limited, few inferences could be drawn from our phylogenies. Our SEM observations showed the detailed morphology of the cephalic extremity, precloacal pseudo-sucker, caudal papillae, phasmids and vulva. sp. n. differs from the other four parasites species of marsupials by the number of caudal papillae and the structure dimensions, and size of the spicule. Moreover, sp. n. has ten pairs of caudal papillae, which is unique compared to other species. We present morphometric and molecular data on this new species, contributing to future studies on subuluroids.
巴西白腹毛足鼠的寄生虫生物多样性仍未被充分探索。我们根据光镜和扫描电子显微镜(SEM)的结果,描述了一种来自白腹毛足鼠大肠的新的(棘头虫目:Subuluroidea)物种。我们还部分测序了新物种的线粒体细胞色素 c 氧化酶 I(MT-CO1)基因,利用分子系统发育分析确定其在 Subuluroidea 超科内的关系。由于有关 subuluroid 物种的分子数据极为有限,我们的系统发育分析只能得出很少的推论。我们的 SEM 观察显示了头部、前肛假吸盘、尾部乳头、phasmids 和阴道的详细形态。新种与其他四种有袋类寄生虫的区别在于尾部乳头的数量和结构尺寸以及尾刺的大小。此外,新种有十对尾部乳头,与其他物种相比是独特的。我们提供了这个新物种的形态和分子数据,为未来的 subuluroid 研究做出了贡献。