Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security, College of Life Science and Engineering, Shenyang University, Dadong, Shenyang, 110044, Liaoning, China.
National Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University, Beibei, Chongqing, 400712, China.
Arch Virol. 2022 Nov;167(11):2403-2405. doi: 10.1007/s00705-022-05569-6. Epub 2022 Aug 9.
A putative new emaravirus, named "ailanthus crinkle leaf-associated emaravirus" (ACrLaV), was detected in Ailanthus altissima with severe crinkle symptoms by RNA-Seq and RT-PCR. Four viral segments associated with ACrLaV were identified and fully sequenced, except for a few nucleotides at the genomic termini. The RNA-dependent RNA polymerase (RNA1), glycoprotein (RNA2), nucleocapsid protein (RNA3), and movement protein (RNA4), showed 26.5%-57%, 17%-49.9%, 14.4%-40.4%, and 14.1%-65.9% amino acid sequence identity, respectively, to those of known emaraviruses. All four ACrLaV genomic RNA segments are most closely related to those of common oak ringspot-associated virus from Germany, as supported by sequence comparisons and phylogenetic analysis. ACrLaV is considered a distinct member of the genus Emaravirus, and this is the first report of an emaravirus in A. altissima.
一种假定的新型埃玛病毒，名为“臭椿皱叶相关埃玛病毒”（ACrLaV），通过 RNA-Seq 和 RT-PCR 在出现严重皱叶症状的臭椿中被检测到。与 ACrLaV 相关的四个病毒片段已被鉴定并进行了全长测序，除了基因组末端的几个核苷酸。RNA 依赖性 RNA 聚合酶（RNA1）、糖蛋白（RNA2）、核衣壳蛋白（RNA3）和运动蛋白（RNA4）的氨基酸序列同一性分别为 26.5%-57%、17%-49.9%、14.4%-40.4%和 14.1%-65.9%，与已知的埃玛病毒相似。序列比较和系统发育分析支持所有四个 ACrLaV 基因组 RNA 片段与德国常见栎树环斑相关病毒的关系最为密切。ACrLaV 被认为是埃玛病毒属的一个独特成员，这是首次在臭椿中发现埃玛病毒。
