Stewart B W, Hristoforidis C
Cancer Lett. 1987 Jun;35(3):263-9. doi: 10.1016/0304-3835(87)90128-5.
Structural analysis, by benzoylated O-(diethylaminoethyl) (DEAE)-cellulose chromatography, was made of DNA from the livers of rats receiving 100 mg/kg diethylnitrosamine and subsequently subjected to partial hepatectomy. Under these conditions, different DNA labelling procedures permit damage to be associated with pre-existing or newly synthesised DNA. Persistent single stranded regions could be detected in DNA isolated more than 3 days after carcinogen treatment only if the animals were subjected to hepatectomy. This damage was attributable to lesions impeding DNA replication. Induction of proliferative activity up to at least 14 days after nitrosamine treatment made manifest DNA damage, the extent of which was not decreased as the interval between carcinogen treatment and surgery was increased.
采用苯甲酰化的O-（二乙氨基乙基）（DEAE）-纤维素色谱法，对接受100mg/kg二乙基亚硝胺并随后接受部分肝切除术的大鼠肝脏DNA进行了结构分析。在这些条件下，不同的DNA标记程序可使损伤与预先存在的或新合成的DNA相关联。仅当动物接受肝切除术后，才能在致癌物处理3天以上分离的DNA中检测到持续的单链区域。这种损伤归因于阻碍DNA复制的损伤。亚硝胺处理后至少14天内增殖活性的诱导使DNA损伤显现出来，随着致癌物处理与手术之间间隔时间的增加，损伤程度并未降低。
