Barsky S H, Gopalakrishna R
Cancer Lett. 1987 Jun;35(3):271-9. doi: 10.1016/0304-3835(87)90129-7.
BL6 melanoma cells injected subcutaneously in 18-month-old syngeneic C57BL/6 mice elicit a marked fibrotic response highly similar in myofibroblast composition and Type V collagen content to that characterizing the desmoplastic response of human carcinomas. This host response can be quantitated in vivo by measuring both hydroxyproline content (total collagen) and incorporation of intraperitoneally injected [14C] proline into collagenase-sensitive protein (new collagen synthesis). 70% inhibition of the response can be achieved with daily L-3,4-dehydroproline. The response can be similarly quantitated in vitro in explants of desmoplastic tumor tissue. The model allows for subsequent investigations of the effects of the desmoplastic response on tumor invasion and metastasis.
将BL6黑色素瘤细胞皮下注射到18月龄同基因C57BL/6小鼠体内，会引发显著的纤维化反应，其肌成纤维细胞组成和Ⅴ型胶原蛋白含量与人类癌的促结缔组织增生反应极为相似。这种宿主反应可通过测量羟脯氨酸含量（总胶原蛋白）以及腹膜内注射的[14C]脯氨酸掺入胶原酶敏感蛋白（新胶原蛋白合成）的情况在体内进行定量。每日使用L-3,4-脱氢脯氨酸可实现70%的反应抑制。在促结缔组织增生性肿瘤组织外植体中，也可在体外进行类似的定量。该模型可用于后续研究促结缔组织增生反应对肿瘤侵袭和转移的影响。
