Laboratoire de Biologie marine (CP 160/15), Université Libre de Bruxelles, Av. F.D. Roosevelt 50, Brussels B-1050, Belgium; Marine Environment Laboratories, International Atomic Energy Agency, 4a, Quai Antoine 1er, Monaco MC-98000, Monaco.
Marine Environment Laboratories, International Atomic Energy Agency, 4a, Quai Antoine 1er, Monaco MC-98000, Monaco.
Aquat Toxicol. 2022 Sep;250:106235. doi: 10.1016/j.aquatox.2022.106235. Epub 2022 Jul 6.
The fate and toxicity of ingested marine microplastics (MPs) have been of major concern in aquatic ecotoxicology for the last decade. Although their ingestion by a wide range of marine organisms has been proven, the uptake of MPs within organs is not yet fully understood and relies on the ability of ingested microplastics to transfer from the gut to tissues beyond the digestive wall (i.e., translocation). The present study investigates the in vitro transfer of fluorescent high-density polyethylene particles of different sizes classes (1-5 µm; 10-29 µm; 38-45 µm) across the intestinal wall of the sea urchin Paracentrotus lividus using Ussing chambers. Small microplastics (1-5 µm) were proven to be able to cross the intestinal wall of P. lividus and reach the coelomic fluid, while larger microplastics (≥ 10 µm) were not observed to cross the intestinal wall. Results demonstrate a size-dependent passage of polyethylene microparticles across the intestinal walls of P. lividus for the first time, highlighting the suitability of Ussing chamber systems to study the transfer of MPs across the intestinal wall of animals.
在过去的十年中，摄入的海洋微塑料（MPs）的命运和毒性一直是水生生态毒理学的主要关注点。尽管已经证明它们被广泛的海洋生物摄入，但 MPs 在器官内的吸收还不完全清楚，并且依赖于摄入的微塑料从肠道转移到消化壁以外的组织（即易位）的能力。本研究使用 Ussing 室调查了不同大小类别（1-5 µm；10-29 µm；38-45 µm）的荧光高密度聚乙烯颗粒在海胆 Paracentrotus lividus 肠壁内的体外转移。已经证明小的微塑料（1-5 µm）能够穿过 P. lividus 的肠壁并到达体腔液，而较大的微塑料（≥ 10 µm）则未观察到穿过肠壁。结果首次证明了聚乙烯微粒在穿过 P. lividus 肠壁时具有尺寸依赖性，突出了 Ussing 室系统在研究 MPs 穿过动物肠壁转移方面的适用性。
