University of Copenhagen, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, DK 1870 Frederiksberg, Denmark.
Chr. Hansen A/S, Animal Health Innovation, Hoersholm, Denmark.
Poult Sci. 2022 Oct;101(10):102029. doi: 10.1016/j.psj.2022.102029. Epub 2022 Jun 26.
In this study, we investigate the effect of the probiotic E. faecium 669 strain on the gut integrity of broilers and the effect on intestinal colonization with Salmonella Enteritidis. In the in vivo experiment, 120-day-old broilers (Ross 308) were divided into 4 equally sized groups. Group A received the probiotic as a single dose by spray at d 18 of incubation and group B received the probiotic in the drinking water daily throughout the experiment. Group C was untreated control. Group D received the antibiotic Apramycin sulfate in the drinking water. Broilers in all four groups were challenged with S. Enteritidis by oral gavage at d 8 of life. From d 9 to 12, a cloacal swab was collected from all broilers for culturing on Salmonella selective media to determine the shedding. At d 12, birds were euthanized and S. Enteritidis in ceca were enumerated and intestinal samples for histology and host gene expression were collected. The group receiving the probiotic in the drinking water shed significantly less S. Enteritidis compared to the untreated control group at all times. The group receiving a single probiotic application before hatch showed a reduced shedding of Salmonella at d 9 and 10. S. Enteritidis was not detected in the ceca of the antimicrobial treated broilers. Histology of jejuni samples and host gene expression showed that intestinal integrity was enhanced by adding probiotic to the drinking water. Overall, the study shows that pre-hatch and daily application of the probiotic strain E. faecium 669 reduces the colonization of broilers with S. Enteritidis and daily application enhances gut integrity. Application of the probiotic E. faecium strain can be recommended as a method to reduce the colonization of broilers with S. Enteritidis and enhance their gut integrity.
在这项研究中，我们研究了益生菌 E. faecium 669 菌株对肉鸡肠道完整性的影响，以及对肠炎沙门氏菌肠道定植的影响。在体内实验中，将 120 日龄的肉鸡（罗斯 308）分为 4 个大小相等的组。A 组在孵化第 18 天通过喷雾接受单一剂量的益生菌，B 组在整个实验过程中每天通过饮水接受益生菌。C 组为未处理对照组。D 组在饮水中接受抗生素硫酸安普霉素。所有 4 组肉鸡在生命第 8 天通过口服灌胃接受肠炎沙门氏菌挑战。从第 9 天到第 12 天，从所有肉鸡的泄殖腔采集粪便拭子，用于在选择性沙门氏菌培养基上进行培养，以确定脱落情况。在第 12 天，对鸟类进行安乐死，并对盲肠中的肠炎沙门氏菌进行计数，并收集肠道样本进行组织学和宿主基因表达分析。与未处理对照组相比，在所有时间点，通过饮水接受益生菌的组的肠炎沙门氏菌脱落量显著减少。在孵化前接受单一益生菌应用的组在第 9 天和第 10 天显示出肠炎沙门氏菌脱落减少。在接受抗菌处理的肉鸡的盲肠中未检测到肠炎沙门氏菌。空肠样本的组织学和宿主基因表达表明，通过在饮水中添加益生菌增强了肠道完整性。总体而言，该研究表明，在孵化前和每天应用益生菌菌株 E. faecium 669 可减少肠炎沙门氏菌对肉鸡的定植，并且每天应用可增强肠道完整性。应用益生菌 E. faecium 菌株可作为减少肠炎沙门氏菌对肉鸡定植并增强其肠道完整性的方法。
