Yang Yuting, Hong Jiajun, Zhang Zheng, Zheng Minghao, Zhao Jingang, Fang Xiaowei, Liang Xiongyan, Liu Jing, Yang Yuying, Tian Guangming, Fang Chun
College of Animal Science and Technology, Yangtze University, Jingzhou 434025, China.
College of Animal Science and Technology, Yangtze University, Jingzhou 434025, China.
Poult Sci. 2024 Dec;103(12):104385. doi: 10.1016/j.psj.2024.104385. Epub 2024 Oct 9.
Lactic acid bacteria (LAB) play a key role in regulating the balance of gut microbiota and serve as a suitable alternative to antibiotics. This study aims to evaluate the characteristics of 2 LAB isolates Lactiplantibacillus plantarum Lp71 (L. plantarum Lp71) and Enterococcus faecium Ef72 (E. faecium Ef72), and their roles in alleviating Salmonella Enteritidis infection. Sixty 1-day-old chicks were randomly divided into 4 groups which treated with or without L. plantarum Lp71 and E. faecium Ef72 mixture for 21 d, and then intestinal samples were collected for gut microbiota analysis, pathological and immunohistochemical analysis at 24 h post infection with or without Salmonella Enteritidis on the 22nd d. The results showed that L. plantarum Lp71 and E. faecium Ef72 had the ability to anti-acid and anti-bile salt. Salmonella Enteritidis infection damaged the intestinal epithelial barrier and reduced the expression level of tight junction proteins (ZO-1, Claudin-1, Occludin). Oral supplementation with L. plantarum Lp71 and E. faecium Ef72 mixture could alleviated the damages to intestinal epithelial barrier by Salmonella Enteritidis infection. Salmonella Enteritidis could cause abnormal Akkermansia muciniphila proliferation and decrease the diversity of cecal microbiota in chicks. These conditions could have further led to reduce gut microbiota health index (GMHI), and improve microbial dysbiosis index (MDI). Moreover, oral supplementation with L. plantarum Lp71 and E. faecium Ef72 mixture could effectively prevent the aforementioned infection outcomes and increase the abundance proportions of the several key functions in metabolic pathways metabolic pathways such as transcription and signal transduction mechanisms. In summary, L. plantarum Lp71 and E. faecium Ef72 could be the probiotics candidates that used to prevent the damage from enteric pathogens such as Salmonella Enteritidis in broiler chicks.
乳酸菌（LAB）在调节肠道微生物群平衡中起关键作用，是抗生素的合适替代品。本研究旨在评估2株乳酸菌分离株植物乳杆菌Lp71（L. plantarum Lp71）和屎肠球菌Ef72（E. faecium Ef72）的特性，以及它们在减轻肠炎沙门氏菌感染中的作用。将60只1日龄雏鸡随机分为4组，用或不用植物乳杆菌Lp71和屎肠球菌Ef72混合物处理21天，然后在第22天感染或不感染肠炎沙门氏菌后24小时收集肠道样本进行肠道微生物群分析、病理和免疫组织化学分析。结果表明，植物乳杆菌Lp71和屎肠球菌Ef72具有抗酸和抗胆盐的能力。肠炎沙门氏菌感染破坏了肠道上皮屏障，降低了紧密连接蛋白（ZO-1、Claudin-1、Occludin）的表达水平。口服植物乳杆菌Lp71和屎肠球菌Ef72混合物可减轻肠炎沙门氏菌感染对肠道上皮屏障的损害。肠炎沙门氏菌可导致雏鸡中嗜黏蛋白阿克曼氏菌异常增殖，并降低盲肠微生物群的多样性。这些情况可能进一步导致肠道微生物群健康指数（GMHI）降低，并改善微生物失调指数（MDI）。此外，口服植物乳杆菌Lp71和屎肠球菌Ef72混合物可有效预防上述感染结果，并增加代谢途径中几个关键功能（如转录和信号转导机制）的丰度比例。总之，植物乳杆菌Lp71和屎肠球菌Ef72可能是用于预防肉鸡雏鸡肠炎沙门氏菌等肠道病原体损害的益生菌候选菌株。
