Department of Biosciences, Integral University, Lucknow, Uttar Pradesh-226026, India.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia.
J Food Biochem. 2022 Oct;46(10):e14368. doi: 10.1111/jfbc.14368. Epub 2022 Aug 9.
Liver cancer or hepatocellular carcinoma (HCC) has become a leading cause for cancer burden across the globe, and incidences have tripled since the last two decades. Poor diagnosis of primary liver cancer and limited treatment strategies aggravate the challenges. Researchers globally have shown a steep inclination toward the exploration of plant-based compounds for their nutraceutical and anticancer potential to fit into the role of novel chemotherapeutics. Coleus aromaticus is a well-known culinary herb that earlier has been reported for several medicinal attributes. The current investigation deals with exploring the anticancer potential of ethanolic leaf extract of C. aromaticus (CoL-EtOH) against hepatocellular carcinoma HepG2 cell line. The observations made it evident that CoL-EtOH extract impeded the viability of HepG2 at 400 μg/ml (p < .01). Additionally, the extract also succeeded in escalating ROS production (p < .01) which aided dissipation of mitochondrial membrane potential and disruption of nuclear morphology. CoL-EtOH further activated caspase-8, -9, and -3 which was reaffirmed by increase in apoptosis at 400 μg/ml (p < .01). Moreover, post treatment with CaLEt-OH extract significantly reduced the expression of JAK-1 & STAT-3 genes (p < .01) along with regulated expression of Mcl1, Bcl-2, cyclinD1, p21, and p27 within HepG2 cells. This evidence portrays the promising anticancer potential of CoL-EtOH projecting it as a novel chemotherapeutic agent against HCC. PRACTICAL APPLICATIONS: The herb Coleus aromaticus belonging to Lamiaceae family and Coleus genus is known by various names in different regions of the world and several language-specific vernacular names. The herb has been used in therapeutic and medicinal applications as well as in culinary preparations. Various attributes of the nutritional strength and functional characteristics of the leaves in terms of carotenoids, minerals, phenols, dietary fiber, and antioxidant activity have been reported by several researchers. Carvacrol and thymol are majorly found in the plant, while chlorogenic acid and rosmarinic acid etc. as the phenolic components. The herb has been used in therapeutic and medicinal implications as well as in culinary preparations.
肝癌或肝细胞癌（HCC）已成为全球癌症负担的主要原因，自过去二十年以来，其发病率增加了两倍。原发性肝癌诊断不佳和治疗策略有限加剧了这些挑战。全球研究人员对植物化合物的探索表现出浓厚的兴趣，因为它们具有营养和抗癌潜力，可以作为新型化疗药物发挥作用。罗勒是一种广为人知的烹饪香草，此前已被报道具有多种药用特性。目前的研究旨在探索罗勒叶乙醇提取物（CoL-EtOH）对肝癌 HepG2 细胞系的抗癌潜力。研究结果表明，CoL-EtOH 提取物在 400μg/ml 时抑制 HepG2 的活力（p<.01）。此外，该提取物还成功地增加了活性氧（ROS）的产生（p<.01），这有助于线粒体膜电位耗散和核形态的破坏。CoL-EtOH 进一步激活了 caspase-8、-9 和 -3，这在 400μg/ml 时通过增加细胞凋亡得到了证实（p<.01）。此外，用 CoLEt-OH 提取物处理后，JAK-1 和 STAT-3 基因的表达显著降低（p<.01），同时调节了 HepG2 细胞中 Mcl1、Bcl-2、cyclinD1、p21 和 p27 的表达。这些证据表明，CoL-EtOH 具有有前途的抗癌潜力，可将其作为治疗 HCC 的新型化疗药物。实际应用：唇形科罗勒属罗勒属植物罗勒属，在世界不同地区和几种特定语言的方言中都有不同的名称。该草药已用于治疗和药用应用以及烹饪准备。几位研究人员报告了叶片在类胡萝卜素、矿物质、酚类化合物、膳食纤维和抗氧化活性方面的各种营养强度和功能特性。该植物中主要含有香芹酚和百里酚，而绿原酸和迷迭香酸等则为酚类成分。该草药已用于治疗和药用应用以及烹饪准备。
