Zhang Yang, Ai Ping, Chen Shang-Zhou, Lei Shu-Ying
Department of Dermatology, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, PR China.
Department of Dermatology, Minda Hospital of Hubei Minzu University, Enshi, PR China.
Immunopharmacol Immunotoxicol. 2023 Feb;45(1):52-60. doi: 10.1080/08923973.2022.2112221. Epub 2022 Aug 22.
The present study aimed to explore the impact of sulforaphane on the growth of sSCC cells, and the activation of miR-199a-5pSirt1 and CD44ICD signaling pathways.
Cell viability, count, apoptosis, and invasion assays were performed in the sSCC cell line (SCC-13) in which miR-199a-5p was over-expressed or under-expressed. The expression levels of miR-199a-5p, Sirt1 and CD44ICD mRNA were measured by quantitative real-time polymerase chain reaction (qRT-PCR).
Sulforaphane significantly inhibited the cell growth and invasion of SCC-13 cells, and dramatically induced cell apoptosis. Additionally, sulforaphane also greatly increased miR-199a-5p expression and suppressed Sirt1 and CD44ICD mRNA levels. Moreover, miR-199a-5p overexpression considerably down-regulated the expressions of Sirt1 and CD44ICD mRNA, and promoted the ability of sulforaphane to represses cell growth and invasion, and to induce cell apoptosis. However, miR-199a-5p underexpression has the opposite effects.
Sulforaphane appears to inhibit sCC progression by impacting its growth and invasion ability, and regulates miR-199a-5p/Sirt1 and CD44ICD signaling pathways, and may be utilized to develop a curative approach for sSCC.
本研究旨在探讨萝卜硫素对皮肤鳞状细胞癌（sSCC）细胞生长以及miR-199a-5p/Sirt1和CD44ICD信号通路激活的影响。
在miR-199a-5p过表达或低表达的sSCC细胞系（SCC-13）中进行细胞活力、计数、凋亡和侵袭实验。通过定量实时聚合酶链反应（qRT-PCR）检测miR-199a-5p、Sirt1和CD44ICD mRNA的表达水平。
萝卜硫素显著抑制SCC-13细胞的生长和侵袭，并显著诱导细胞凋亡。此外，萝卜硫素还大幅增加miR-199a-5p的表达，并抑制Sirt1和CD44ICD mRNA水平。而且，miR-199a-5p过表达显著下调Sirt1和CD44ICD mRNA的表达，并增强萝卜硫素抑制细胞生长和侵袭以及诱导细胞凋亡的能力。然而，miR-199a-5p低表达则产生相反的效果。
萝卜硫素似乎通过影响sSCC的生长和侵袭能力来抑制其进展，并调节miR-199a-5p/Sirt1和CD44ICD信号通路，可能可用于开发sSCC的治疗方法。
