Du Yujie, Li Yajuan, Tian Zhonghuan, Cheng Yunjiang, Long Chao-An
Key Laboratory of Horticultural Plant Biology of Ministry of Education, National R&D Center For Citrus Preservation, National Centre of Citrus Breeding, Huazhong Agricultural University, Wuhan, China.
J Appl Microbiol. 2022 Dec;133(6):3438-3450. doi: 10.1111/jam.15769. Epub 2022 Aug 31.
The purpose of this study was to explore the potential inhibitory mechanism and assess the feasibility of natamycin as an antifungal agent in the utilization of citrus storage.
In this study, the mycelial growth, spore germination as well as germ tube elongations of Geotrichum citri-aurantii and Penicillium digitatum were significantly inhibited by natamycin treatment. The relative conductivities of G. citri-aurantii and P. digitatum mycelia were increased as time went by and the damages of plasma membranes were up to 17.43% and 28.61%. The mitochondria abnormalities and vacuolation were also observed in the TEM. Moreover, the sour rot and green mould decay incidences were reduced to 18.33% and 10% post incubation with G. citri-aurantii and P. digitatum under 300 mg L natamycin application, respectively. For the citrus storage experiment, there was no significant difference in edible rate, juice yield, total soluble solid (TSS) content, titratable acid (TA) and decay incidences of the 'Newhall' navel orange fruit treated with 300 mg L natamycin stored for 90 d.
Natamycin could decrease the expansions of green mould and sour rot and maintain quality and improve storability on citrus fruit.
This work explores the potential inhibition mechanism of natamycin G. citri-aurantii and P. digitatum and assesses the feasibility of natamycin as an antifungal agent in the utilization of citrus storage.
本研究旨在探索纳他霉素在柑橘贮藏应用中作为抗真菌剂的潜在抑制机制，并评估其可行性。
本研究中，纳他霉素处理显著抑制了橘青霉和指状青霉的菌丝生长、孢子萌发以及芽管伸长。随着时间推移，橘青霉和指状青霉菌丝体的相对电导率增加，质膜损伤分别高达17.43%和28.61%。透射电镜观察还发现线粒体异常和液泡化现象。此外，在300 mg/L纳他霉素处理下，接种橘青霉和指状青霉后，酸腐病和绿霉病发病率分别降至18.33%和10%。对于柑橘贮藏实验，用300 mg/L纳他霉素处理的‘纽荷尔’脐橙果实贮藏90 d后，其可食率、出汁率、总可溶性固形物（TSS）含量、可滴定酸（TA）和腐烂发病率均无显著差异。
纳他霉素可减少绿霉病和酸腐病的扩展，保持柑橘果实品质并提高其耐贮性。
本研究探索了纳他霉素对橘青霉和指状青霉的潜在抑制机制，并评估了纳他霉素在柑橘贮藏应用中作为抗真菌剂的可行性。
