College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, China.
Collaborative Innovation Center of Provincial and Ministerial Co-construction for Marine Food Deep Processing, Dalian Polytechnic University, Dalian 116034, China.
J Agric Food Chem. 2022 Aug 24;70(33):10229-10238. doi: 10.1021/acs.jafc.2c04685. Epub 2022 Aug 10.
This study aimed to determine whether fucoxanthin alleviated ovalbumin (OVA)-induced food allergy (FA) and explored the possible mechanisms. The results indicated that supplementation with fucoxanthin at 10.0-20.0 mg/kg per day for 7 weeks inhibited food anaphylaxis and the production of immunoglobulin (Ig) E, IgG, histamine, and related cytokines while alleviating allergic symptoms in sensitized mice. Fucoxanthin enhanced the intestinal epithelial barrier by up-regulating tight junction (TJ) protein expression and promoting regenerating islet-derived protein III-gamma (RegIIIγ) and secretory IgA (sIgA) secretion. In addition, fucoxanthin induced the secretion of anti-inflammatory factors (interleukin (IL)-10 and transforming growth factor β (TGF-β)) by regulatory T (Treg) cells and decreased the pro-inflammatory factor levels (IL-4, tumor necrosis factor-α (TNF-α), IL-17, and IL-1β), ameliorating intestinal inflammation. Compared with the model group, beneficial bacteria, such as Lactobacillaceae, increased in the intestinal flora, while pathogenic bacteria like Helicobacteraceae, Desulfovibrionaceae, and Streptococcaceae decreased. Therefore, fucoxanthin may effectively prevent FA by enhancing the intestinal epithelial barrier and reshaping the intestinal flora.
本研究旨在确定岩藻黄质是否能缓解卵清蛋白（OVA）诱导的食物过敏（FA），并探讨其可能的机制。结果表明，岩藻黄质以 10.0-20.0mg/kg/天的剂量补充 7 周，可抑制食物过敏反应和免疫球蛋白（Ig）E、IgG、组胺和相关细胞因子的产生，同时缓解致敏小鼠的过敏症状。岩藻黄质通过上调紧密连接（TJ）蛋白表达和促进再生胰岛衍生蛋白 III-γ（RegIIIγ）和分泌型免疫球蛋白 A（sIgA）分泌来增强肠道上皮屏障。此外，岩藻黄质通过调节性 T（Treg）细胞诱导抗炎因子（白细胞介素（IL）-10 和转化生长因子β（TGF-β））的分泌，并降低促炎因子水平（IL-4、肿瘤坏死因子-α（TNF-α）、IL-17 和 IL-1β），改善肠道炎症。与模型组相比，肠道菌群中有益菌如乳杆菌科增加，而致病菌如螺杆菌科、脱硫弧菌科和链球菌科减少。因此，岩藻黄质可能通过增强肠道上皮屏障和重塑肠道菌群来有效预防 FA。
