Pelc L R, Gross G J, Warltier D C
Circulation. 1987 Jul;76(1):191-200. doi: 10.1161/01.cir.76.1.191.
The transmural distribution of myocardial blood flow across the left ventricles of anesthetized dogs was measured with radioactive microspheres during intracoronary infusion of the endothelium-dependent vasodilators acetylcholine (10 micrograms/min), adenosine triphosphate (ATP; 20 micrograms/min), and arachidonic acid (600 micrograms/min) and the endothelium-independent vasodilator nifedipine (5 micrograms/min). These compounds were administered before and after a 30 min intracoronary infusion of the phospholipase A2 inhibitor quinacrine (300 micrograms/min). Acetylcholine, ATP, and arachidonic acid produced significant (p less than .05) increases in transmural blood flow and in the ratio of subendocardial to subepicardial blood flow (endo/epi) when compared with control. Infusion of quinacrine did not affect this ratio and did not block the increase in transmural blood flow produced by each agent; however, it did block the redistribution of flow to the subendocardium. In contrast, there was no change in endo/epi during intracoronary infusion of nifedipine before and after quinacrine. These results suggest that endothelium-dependent vasodilators produce a preferential increase in subendocardial perfusion via a product of unsaturated fatty acid metabolism.
在冠状动脉内输注内皮依赖性血管舒张剂乙酰胆碱（10微克/分钟）、三磷酸腺苷（ATP；20微克/分钟）和花生四烯酸（600微克/分钟）以及内皮非依赖性血管舒张剂硝苯地平（5微克/分钟）期间，用放射性微球测量了麻醉犬左心室心肌血流的透壁分布。这些化合物在冠状动脉内输注磷脂酶A2抑制剂奎纳克林（300微克/分钟）30分钟之前和之后给药。与对照组相比，乙酰胆碱、ATP和花生四烯酸使透壁血流以及心内膜下与心外膜下血流之比（endo/epi）显著（p<0.05）增加。输注奎纳克林不影响该比值，也不阻断每种药物引起的透壁血流增加；然而，它确实阻断了血流向内膜下的重新分布。相比之下，在冠状动脉内输注硝苯地平期间，奎纳克林前后endo/epi没有变化。这些结果表明，内皮依赖性血管舒张剂通过不饱和脂肪酸代谢产物使心内膜下灌注优先增加。
