Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, University of Nigeria, Enugu Campus.
Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, University of Nigeria, Enugu Campus .
Niger J Physiol Sci. 2022 Jun 30;37(1):113-117. doi: 10.54548/njps.v37i1.14.
Hibiscus sabdariffa (HS) has gained attention as an anti-hypertensive agent. In the present study, we hypothesized that anthocyanins from HS may attenuate salt-induced hypertension in rats by suppressing the components of renin-angiotensin-aldoslestrone system (RAAS). Hypertension was induced in the rats by adding 8% NaCl in their diet for six weeks. Wistar rats (n=5 each) were randomly divided into seven groups. Group 1 was the normentensive control group and was fed with normal rat chew and water ad libitum; groups 2 and 3 served as hypertensive control (negative untreated and positive treated with captopril 30mg/kg respectively); groups 4, 5, and 6 served as treatment groups and were administered with graded doses of anthocyanins( 50, 100, 200mg/kg respectively) while group 7 received both 100mg anthocyanins and 30mg captopril per day for 4 weeks. Using HPLC, anthocyanins were isolated from HS calyx following standard protocol. Anthocyanins significantly (p<0.05) reduced blood pressure and heart rate in hypertensive rats in a dose dependent manner. The blood pressure reduction by anthocyanins was associated with a reduction in serum ACE and plasma aldosterone in the hypertensive rats. The effects of anthocyanins on blood pressure and on biomarkers of RAAS were similar to those of captopril, a reference anti-hypertensive drug. The results suggest that anthocyanins exerts a significant (p<0.05) anti-hypertensive potency on rats, probably mediated by the reduction in components of the RAAS. Keywords: hypertension, anthocyanins, renin, aldosterone, rats.
玫瑰茄（HS）作为一种抗高血压药物引起了人们的关注。在本研究中，我们假设 HS 的花色苷可能通过抑制肾素-血管紧张素-醛固酮系统（RAAS）的成分来减轻盐诱导的高血压。通过在大鼠饮食中添加 8%的 NaCl 来诱导高血压，持续 6 周。将 Wistar 大鼠（每组 5 只）随机分为 7 组。第 1 组为正常血压对照组，给予正常大鼠咀嚼物和自由饮水；第 2 组和第 3 组为高血压对照组（未治疗的阴性对照和用卡托普利 30mg/kg 治疗的阳性对照）；第 4、5、6 组为治疗组，分别给予不同剂量的花色苷（50、100、200mg/kg），第 7 组同时给予 100mg 花色苷和 30mg 卡托普利，持续 4 周。采用 HPLC 法，按照标准方案从 HS 花萼中分离花色苷。花色苷显著（p<0.05）降低了高血压大鼠的血压和心率，呈剂量依赖性。花色苷降低血压与高血压大鼠血清 ACE 和血浆醛固酮的降低有关。花色苷对血压和 RAAS 生物标志物的作用与参考抗高血压药物卡托普利相似。结果表明，花色苷对大鼠具有显著的（p<0.05）降压作用，可能是通过降低 RAAS 的成分介导的。关键词：高血压，花色苷，肾素，醛固酮，大鼠。
