qnrVC 存在于从巴西沿海水域分离的克雷伯氏菌和肠杆菌菌株的不同遗传环境中。
qnrVC occurs in different genetic contexts in Klebsiella and Enterobacter strains isolated from Brazilian coastal waters.
机构信息
Laboratório de Investigação em Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Departamento de Zootecnia, Universidade Federal da Paraíba, Areia, Paraíba, Brazil.
出版信息
J Glob Antimicrob Resist. 2022 Dec;31:38-44. doi: 10.1016/j.jgar.2022.08.004. Epub 2022 Aug 7.
OBJECTIVES
In contrast to other qnr families, qnrVC has been reported mainly in Vibrio spp. and inserted in class 1 integrons. This study aimed to identify the variants of qnrVC genes detected in Klebsiella pneumoniae carbapenemase-2-producing Enterobacter and Klebsiella strains isolated from Brazilian coastal waters and the genetic contexts associated with their occurrence.
METHODS
qnrVC variants were identified by Sanger sequencing. Stains were typified by pulsed-field gel electrophoresis. Antimicrobial susceptibility testing, conjugation assays, and whole genome sequencing (WGS) were applied to identify the strains' antimicrobial resistance profile, qnrVC and bla co-transference, and qnrVC genetic context.
RESULTS
qnrVC1 was identified in 15 Enterobacter and 3 Klebsiella, and qnrVC4 in 2 Enterobacter strains. Pulsed-field gel electrophoresis revealed 12 clonal profiles of Enterobacter and one of Klebsiella. Strains were resistant to aminoglycosides, beta-lactams, fosfomycin, quinolones, and sulfamethoxazole-trimethoprim. Co-transference of qnrVC and bla were obtained from five representative Enterobacter strains, which showed resistance to ampicillin and amoxicillin-clavulanate, and reduced susceptibility to extended-spectrum cephalosporins, meropenem, and ciprofloxacin. WGS analysis from representative strains revealed one K. quasipneumoniae subsp. similipneumoniae, one E. soli, four E. kobei, and seven isolates belonging to Enterobacter Taxon 3. Long-read WGS showed qnrVC and bla were carried by the same replicon on Klebsiella and Enterobacter strains, and the qnrVC association with not previously described genetic environments composed of insertion sequences and truncated genes. These contexts occurred in small- and high-molecular-weight plasmids belonging to IncFII, IncP6, pKPC-CAV1321, and IncU groups.
CONCLUSION
Our results suggest that the dissemination of qnrVC among Enterobacterales in Brazilian coastal waters is associated with several genetic recombination events.
目的
与其他 qnr 家族不同，qnrVC 主要在弧菌属和插入类 1 整合子中报道。本研究旨在鉴定从巴西沿海水域分离的产碳青霉烯酶肠杆菌和克雷伯氏菌中检测到的 qnrVC 基因的变体，以及与其发生相关的遗传环境。
方法
通过 Sanger 测序鉴定 qnrVC 变体。利用脉冲场凝胶电泳对菌株进行分型。应用抗菌药物敏感性试验、接合试验和全基因组测序（WGS）来鉴定菌株的抗菌药物耐药谱、qnrVC 和 bla 共转移以及 qnrVC 遗传环境。
结果
在 15 株肠杆菌和 3 株克雷伯氏菌中鉴定出 qnrVC1，在 2 株肠杆菌中鉴定出 qnrVC4。脉冲场凝胶电泳显示肠杆菌有 12 种克隆谱，克雷伯氏菌有 1 种。菌株对氨基糖苷类、β-内酰胺类、磷霉素、喹诺酮类和磺胺甲噁唑-甲氧苄啶耐药。从 5 株代表性肠杆菌中获得了 qnrVC 和 bla 的共转移，这些菌株对氨苄西林和阿莫西林-克拉维酸耐药，对头孢菌素、美罗培南和环丙沙星的敏感性降低。代表性菌株的 WGS 分析显示，1 株肺炎克雷伯菌亚种相似亚种，1 株 E. soli，4 株 E. kobei 和 7 株属于肠杆菌科分类 3 的分离株。长读 WGS 显示 qnrVC 和 bla 由 Klebsiella 和 Enterobacter 菌株上的相同复制子携带，并且 qnrVC 与以前未描述的由插入序列和截短基因组成的遗传环境相关联。这些环境发生在属于 IncFII、IncP6、pKPC-CAV1321 和 IncU 组的小分子量和高分子量质粒中。
结论
我们的结果表明，巴西沿海水域肠杆菌科中 qnrVC 的传播与几种遗传重组事件有关。
Zotero 插件