Puopolo P R, Flood J G
Clin Chem. 1987 Jun;33(6):819-20.
We evaluated a technique for detecting cyclobenzaprine interference with liquid-chromatographic assays for tricyclic antidepressants. The technique involves dual-wavelength absorbance monitoring of the column effluent at 214 and 254 nm. Ratios of analyte peak heights at each wavelength are used to check for the presence of co-eluting interferences. With this technique, one can detect interference with an amitriptyline assay caused by 10 micrograms of cyclobenzaprine per liter.
我们评估了一种检测环苯扎林对三环类抗抑郁药液相色谱测定干扰的技术。该技术包括在214和254nm处对柱流出物进行双波长吸光度监测。利用每个波长处分析物峰高的比值来检查是否存在共洗脱干扰。采用该技术，能够检测出每升含10微克环苯扎林时对阿米替林测定的干扰。
