Pan Xinyuan, Hui Huang, Teng Xiaopin, Wei Kuicheng
Department of Plastic Surgery, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China.
Department of Orthopedics, Minzu Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi Zhuang Autonomous Region 530001, P.R. China.
Mol Clin Oncol. 2022 Jul 12;17(3):133. doi: 10.3892/mco.2022.2566. eCollection 2022 Sep.
Infantile hemangioma is a common benign tumor in infants. However, the molecular mechanism that controls the proliferation and differentiation of hemangioma is not well understood. Annexin A1 (ANX A1) is a phospholipid-binding protein involved in a variety of biological processes, including inflammation, cell proliferation and apoptosis. To explore the significance of ANX A1 in the process of proliferation or differentiation of hemangioma, proliferating and involuting hemangioma tissues were collected to detect the expression of ANX A1 using immunohistochemistry and western blotting. Normal skin tissues were used as the negative control. The results revealed that ANX A1 was upregulated in the proliferative phase of hemangioma, and its expression was decreased when the hemangioma entered the involuting phase. Additionally, in the proliferative phase, the strongest staining of ANX A1 was observed in newly born capillaries, and the staining of ANX A1 became weaker in enlarged vessels, indicating that ANX A1 plays an important role in promoting the formation of capillaries. The expression of hypoxia-inducible factor (HIF)-1α was positively associated with the expression trend of ANX A1, suggesting that the overexpression of ANX A1 may be associated with the increase of HIF-1α. In summary, the results of the present study revealed that the expression of ANX A1 was increased in proliferating hemangioma tissue, and that high expression of ANX A1 may be closely associated with the formation of capillaries in infantile hemangioma.
婴儿血管瘤是婴儿常见的良性肿瘤。然而，控制血管瘤增殖和分化的分子机制尚不清楚。膜联蛋白A1（ANX A1）是一种磷脂结合蛋白，参与多种生物学过程，包括炎症、细胞增殖和凋亡。为了探讨ANX A1在血管瘤增殖或分化过程中的意义，收集增殖期和消退期的血管瘤组织，采用免疫组织化学和蛋白质印迹法检测ANX A1的表达。正常皮肤组织作为阴性对照。结果显示，ANX A1在血管瘤增殖期上调，当血管瘤进入消退期时其表达降低。此外，在增殖期，新生毛细血管中观察到ANX A1染色最强，而在扩张血管中ANX A1染色变弱，表明ANX A1在促进毛细血管形成中起重要作用。缺氧诱导因子（HIF）-1α的表达与ANX A1的表达趋势呈正相关，提示ANX A1的过表达可能与HIF-1α的增加有关。总之，本研究结果显示，增殖期血管瘤组织中ANX A1表达增加，且ANX A1的高表达可能与婴儿血管瘤中毛细血管的形成密切相关。
