Askari Masoumeh, Afshar Mohammad, Naghizadeh Ali, Khorashadizadeh Mohsen, Zardast Mahmoud
Department of Anatomy, Faculty of Medicine, 125609Birjand University of Medical Sciences, Birjand, Iran.
Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Int J Low Extrem Wounds. 2022 Aug 11:15347346221118497. doi: 10.1177/15347346221118497.
In recent years, nanotechnology and the subsequent production of nanoparticles have developed excellent methods for medical applications, including wound healing. One of these nanoparticles is bentonite nanoparticles (BNPs) which show high ability in tissue engineering. But our knowledge of its effectiveness in wound healing is based on little data. Therefore, the main purpose of this study was to evaluate the wound healing ability of BNPs and in the next step the suitability of honey as a solvent for these nanoparticles. In this experimental study, an excisional wound injury model was developed in adult male BALB/c mice (n = 60) by creative two equal-sized wounds (5 mm) on either side of their back midline. The animals were allocated into five groups (n = 12 each) as untreated control (U), honey (H), polyethylene glycol (P), and (BNPs) dissolved in honey or polyethylene glycol (H + BNPs, P + BNPs). Animals have received their relative topical treatments twice per day for 14 consecutive days. Tissue sampling was carried out on days 4, 7, 10, and 14. The tissue sections were stained with hematoxylin and eosin and Trichrome-Masson staining methods. The histomorphological parameters including inflammatory cells infiltration, fibroblasts, re-epithelialization, granulation formation, and collagenases were evaluated in all tissue sections. Data were analyzed by SPSS 16 software. Comparison between the groups was performed by one-way analysis of variance following Tukey's post-hoc test. Compared to the control group, BNPs showed significant wound healing activities with lower inflammatory cells infiltration, higher fibroblastosis and new epithelium thickness, and greater granulation area and collagen fibers density in the ulcer bed. In addition, honey as a solvent synergistically increased the wound healing activity of the studied nanoparticle. These results for the first time are clearly showing that BNPs have a promising wound healing activity, especially when applied with honey concurrently.
近年来，纳米技术及随后的纳米颗粒生产为包括伤口愈合在内的医学应用开发了出色的方法。这些纳米颗粒之一是膨润土纳米颗粒（BNPs），其在组织工程中显示出很高的能力。但我们对其在伤口愈合方面有效性的了解基于很少的数据。因此，本研究的主要目的是评估BNPs的伤口愈合能力，接下来评估蜂蜜作为这些纳米颗粒溶剂的适用性。在本实验研究中，通过在成年雄性BALB/c小鼠（n = 60）背部中线两侧制造两个大小相等的伤口（5毫米），建立了切除性伤口损伤模型。将动物分为五组（每组n = 12），即未处理对照组（U）、蜂蜜组（H）、聚乙二醇组（P）以及溶解在蜂蜜或聚乙二醇中的（BNPs）组（H + BNPs、P + BNPs）。动物连续14天每天接受两次相对的局部治疗。在第4、7、10和14天进行组织取样。组织切片用苏木精和伊红染色以及三色-马森染色法染色。在所有组织切片中评估包括炎性细胞浸润、成纤维细胞、再上皮化、肉芽形成和胶原酶在内的组织形态学参数。数据用SPSS 16软件进行分析。组间比较采用单因素方差分析，随后进行Tukey事后检验。与对照组相比，BNPs显示出显著的伤口愈合活性，炎性细胞浸润减少、成纤维细胞增生和新上皮厚度增加、溃疡床肉芽面积和胶原纤维密度更大。此外，蜂蜜作为溶剂协同增强了所研究纳米颗粒的伤口愈合活性。这些结果首次清楚地表明，BNPs具有有前景的伤口愈合活性，尤其是与蜂蜜同时应用时。
