Department of Rheumatology and Immunology, Peking University People's Hospital, Beijing, China.
Department of Rheumatology, The Second Hospital of Shanxi Medical University, Beijing, China.
Clin Exp Immunol. 2022 Sep 29;209(3):270-279. doi: 10.1093/cei/uxac071.
To investigate the expression and roles of TAM (Tyro3/Axl/Mer) receptor tyrosine kinases (TK) in synovial fluid and synovial tissue of patients with rheumatoid arthritis (RA). The expression of TAM TKs in the synovial fluid and synovial tissues of RA and osteoarthritis (OA) patients was measured by ELISA and immunohistochemistry. The relationships between soluble TAM TKs (sTAM TKs) levels and the clinical features, laboratory parameters and disease activity were analyzed in RA. The concentrations of sTAM TK in the synovial fluids of RA patients were increased in comparison to those of OA patients. Compared with OA patients, the expression of membrane Tyro3 TK (mTyro3 TK) and mMer TK in RA patient synovial tissue were significantly increased, which may partly explain the possible mechanism of elevated levels of sTAM TK in RA patient synovial fluid. sAxl TK levels were decreased in RA patients under sulfasalazine treatment and elevated in patients under Iguratimod treatment. Furthermore, sTyro3 TK levels were positively correlated with erythrocyte sedimentation rate (ESR) and negatively correlated with white blood cells (WBCs), red blood cells (RBCs), and hemoglobin (HB) in RA patients. The levels of sMer TK were positively associated with disease duration and rheumatoid factor (RF) and negatively correlated with HB, complement 3 (C3), and C4. Taken together, TAM TKs might be involved in RA synovial tissue inflammation.
为了研究 TAM（Tyro3/Axl/Mer）受体酪氨酸激酶（TK）在类风湿关节炎（RA）患者滑液和滑膜组织中的表达和作用。采用 ELISA 和免疫组织化学法检测 RA 和骨关节炎（OA）患者滑液和滑膜组织中 TAM TK 的表达。分析 RA 患者可溶性 TAM TK（sTAM TK）水平与临床特征、实验室参数和疾病活动的关系。与 OA 患者相比，RA 患者滑液中 sTAM TK 的浓度升高。与 OA 患者相比，RA 患者滑膜组织中膜 Tyro3 TK（mTyro3 TK）和 mMer TK 的表达明显增加，这可能部分解释了 RA 患者滑液中 sTAM TK 水平升高的可能机制。柳氮磺胺吡啶治疗的 RA 患者 sAxl TK 水平降低，来氟米特治疗的 RA 患者 sAxl TK 水平升高。此外，RA 患者 sTyro3 TK 水平与红细胞沉降率（ESR）呈正相关，与白细胞（WBC）、红细胞（RBC）和血红蛋白（HB）呈负相关。sMer TK 水平与疾病持续时间和类风湿因子（RF）呈正相关，与 HB、补体 3（C3）和 C4 呈负相关。综上所述，TAM TK 可能参与 RA 滑膜组织炎症。
