类风湿关节炎、银屑病关节炎和骨关节炎中抗环瓜氨酸肽抗体和 IgA 类风湿因子的滑液水平。

Synovial fluid levels of anti-cyclic citrullinated peptide antibodies and IgA rheumatoid factor in rheumatoid arthritis, psoriatic arthritis, and osteoarthritis.

作者信息

Caspi Dan, Anouk Marina, Golan Itzhak, Paran Daphna, Kaufman Ilana, Wigler Irena, Levartovsky David, Litinsky Irena, Elkayam Ori

机构信息

Tel Aviv Sourasky Medical Center and the Sackler Faculty of Medicine, University of Tel Aviv, Tel Aviv 64239, Israel.

出版信息

Arthritis Rheum. 2006 Feb 15;55(1):53-6. doi: 10.1002/art.21691.

DOI:10.1002/art.21691

PMID:16463412

Abstract

OBJECTIVE

To assess the levels of anti-cyclic citrullinated peptide (anti-CCP) and IgA rheumatoid factor (IgA-RF) in synovial fluids of patients with rheumatoid arthritis (RA), psoriatic arthritis (PsA), and osteoarthritis (OA).

METHODS

Knee effusions of 29 patients with RA (23 women, 6 men; mean +/- SD age 60 +/- 15 years), 20 with PsA (6 women, 14 men; mean age 51 +/- 12 years), and 19 with OA (9 women, 10 men; mean age 73 +/- 11.8 years) were aspirated, tested for white blood cell (WBC) counts, centrifuged, and stored at -20 degrees . Sera of 22, 11, and 12 of these patients with RA, PsA, and OA, respectively, were similarly stored. IgG anti-CCP and IgA-RF were detected by enzyme-linked immunosorbent assay. Erythrocyte sedimentation rate and C-reactive protein levels were used as measures of disease activity.

RESULTS

Mean levels of synovial fluid anti-CCP and IgA-RF were significantly increased in RA joint effusions compared with PsA and OA (anti-CCP: 150 +/- 134, 34 +/- 29, and 24 +/- 26 units, respectively [P < 0.003]; IgA-RF: 76 +/- 77, 15.7 +/- 10, and 18 +/- 20 units, respectively). No significant difference was noted between OA and PsA. A significant correlation was found between synovial fluid anti-CCP and serum anti-CCP and IgA-RF. In patients with RA, a significant correlation was found between synovial fluid WBC counts and IgA-RF (P = 0.03) and serum IgA-RF (P = 0.008), but not between synovial fluid and serum anti-CCP levels. In RA patients, C-reactive protein correlated with serum IgA-RF.

CONCLUSION

Anti-CCP and IgA-RF were significantly increased in synovial fluid of RA in comparison with PsA and OA patients.

摘要

目的

评估类风湿关节炎（RA）、银屑病关节炎（PsA）和骨关节炎（OA）患者滑液中抗环瓜氨酸肽（抗CCP）和IgA类风湿因子（IgA-RF）的水平。

方法

抽取29例RA患者（23例女性，6例男性；平均±标准差年龄60±15岁）、20例PsA患者（6例女性，14例男性；平均年龄51±12岁）和19例OA患者（9例女性，10例男性；平均年龄73±11.8岁）的膝关节积液，检测白细胞（WBC）计数，离心后储存在-20℃。这些RA、PsA和OA患者分别有22例、11例和12例的血清也进行同样储存。采用酶联免疫吸附测定法检测IgG抗CCP和IgA-RF。红细胞沉降率和C反应蛋白水平用作疾病活动度的指标。

结果

与PsA和OA相比，RA关节积液中滑液抗CCP和IgA-RF的平均水平显著升高（抗CCP：分别为150±134、34±29和24±26单位[P<0.003]；IgA-RF：分别为76±77、15.7±10和18±20单位）。OA和PsA之间未发现显著差异。滑液抗CCP与血清抗CCP和IgA-RF之间存在显著相关性。在RA患者中，滑液WBC计数与IgA-RF（P=0.03）和血清IgA-RF（P=0.008）之间存在显著相关性，但滑液和血清抗CCP水平之间无相关性。在RA患者中，C反应蛋白与血清IgA-RF相关。