Pathobiology Group, Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine and Zootechnics, Universidad Nacional de Colombia (UN), Bogotá D.C., Colombia.
Laboratory of Veterinary Pathology, Universidad de Ciencias Aplicadas y Ambientales, Bogotá D.C., Colombia.
Res Vet Sci. 2022 Dec 20;152:115-126. doi: 10.1016/j.rvsc.2022.07.026. Epub 2022 Aug 2.
In order to characterize the in vivo lesions in the nasal cavities and lungs, twenty-eight rabbits were intranasally instilled with lipopolysaccharide (LPS) from P. multocida and then divided into seven groups according to euthanasia time. The nasal cavities and the lungs were processed for light microscopy, lectin histochemistry and transmission electron microscopy. Increased goblet cell activation and neutrophil infiltration were relevant changes in the nasal cavity. A predominantly interstitial pattern of diffuse alveolar damage and bronchopneumonic foci were the main lesions found in the lungs. LPS was found in the cytoplasm of ciliated cells, goblet cells, glandular cells, venular endothelial cells and neutrophils in the nasal cavity and in club cells, capillary endothelial cells and neutrophil in the lung. This study demonstrates that the LPS is able to cause lesions in the upper and lower respiratory tract, it binds to and is internalized by respiratory epithelial cells. Furthermore, it also traverses the intercellular spaces to reach the blood vessels, where it binds to and is internalized by neutrophil and red blood cells. These cells may then travel to the lungs where the LPS induces typical diffuse alveolar damage. This route of lung interstitial damage, to our knowledge, has not been described for this molecule or any known pathogen.
为了描述鼻腔和肺部的活体损伤，将 28 只兔子经鼻腔内滴注多杀性巴氏杆菌脂多糖（LPS），然后根据安乐死时间分为 7 组。对鼻腔和肺进行光镜、凝集素组织化学和透射电镜检查。鼻腔中，杯状细胞激活和中性粒细胞浸润增加是相关的变化。弥漫性肺泡损伤和支气管肺炎灶的主要间质性模式是肺部的主要病变。LPS 在鼻腔中的纤毛细胞、杯状细胞、腺细胞、静脉内皮细胞和中性粒细胞的细胞质中，以及在肺中的 club 细胞、毛细血管内皮细胞和中性粒细胞中被发现。本研究表明，LPS 能够引起上呼吸道和下呼吸道的损伤，它与呼吸上皮细胞结合并被内吞。此外，它还穿过细胞间隙到达血管，与中性粒细胞和红细胞结合并被内吞。这些细胞可能随后迁移到肺部，LPS 在肺部引起典型的弥漫性肺泡损伤。据我们所知，对于这种分子或任何已知的病原体，尚未描述这种肺部间质损伤的途径。
