DNA 条形码在整个中药产业链中的应用：以大黄根茎为例。
Application of DNA barcoding to the entire traditional Chinese medicine industrial chain: A case study of Rhei Radix et Rhizoma.
机构信息
Key Laboratory of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Engineering Research Center of Chinese Medicine Resource of Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Key Laboratory of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Engineering Research Center of Chinese Medicine Resource of Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China; School of Life and Science, Southwest Jiaotong University, Chengdu 610031, China.
出版信息
Phytomedicine. 2022 Oct;105:154375. doi: 10.1016/j.phymed.2022.154375. Epub 2022 Aug 2.
BACKGROUND
Safety concerns, caused by complex and unpredictable adulterants, run through the entire industrial chain of traditional Chinese medicines (TCMs). However, the conventional circulation traceability system only focuses on a certain end or link at the back end of the TCM industrial chain, ignoring the integrity of the links cross the entire industrial chain and lacking traceability. In consequence, a strict and rational supervision system is urgently required for the entire industrial chain.
HYPOTHESIS/PURPOSE: We hypothesize that DNA barcoding would be a suitable measure for the traceability of adulterants in the entire TCM industrial chain.
METHODS
In this study, Rhei Radix et Rhizoma was selected as a model to establish a traceability system for the entire TCM industrial chain. A total of 110 samples, including leaves, seeds, roots, decoction pieces, and traditional Chinese patent medicines (TCPMs), were collected upstream, midstream, and downstream of the entire industrial chain of Rhei Radix et Rhizoma. The ndhF-rpl32 fragment rather than the universal DNA barcodes, which could not distinguish the three original species of Rhei Radix et Rhizoma, was selected as a specific DNA barcode to evaluate the practical application of DNA barcoding in the chain.
RESULTS
The results showed that the ndhF-rpl32 fragment in all samples could be amplified and bi-directionally sequenced. Based on the standard operating procedures of DNA barcoding, the ndhF-rpl32 fragment clearly distinguished the seven Rheum species collected upstream of the entire industrial chain. For the samples collected midstream and downstream of the entire industrial chain, 25% of the 36 commercial decoction pieces samples were identified as adulterants, whereas the eight TCPM samples were all derived from genuine Rhei Radix et Rhizoma.
CONCLUSIONS
This study shows that DNA barcoding is a powerful and suitable technology that can be applied to trace TCMs in the entire industrial chain, thereby assuring clinical medication safety.
背景
由于复杂且不可预测的掺杂物，安全性问题贯穿于整个中药（TCM）产业链。然而，传统的流通可追溯性系统仅关注 TCM 产业链后端的某个末端或环节，忽略了整个产业链中各环节的完整性，缺乏可追溯性。因此，迫切需要建立一个严格合理的整个产业链监管体系。
假设/目的：我们假设 DNA 条形码将是整个 TCM 产业链中掺杂物可追溯性的合适措施。
方法
在这项研究中，选择大黄根茎作为模型，建立整个 TCM 产业链的可追溯性系统。共采集了 110 个样品，包括大黄根茎的叶、种子、根、饮片和中药复方（TCPM），这些样品来自整个大黄根茎产业链的上游、中游和下游。选择不能区分大黄根茎的三个原种的通用 DNA 条形码的 ndhF-rpl32 片段作为特定的 DNA 条形码，以评估 DNA 条形码在链条中的实际应用。
结果
结果表明，所有样品的 ndhF-rpl32 片段都可以扩增和双向测序。基于 DNA 条形码的标准操作程序，ndhF-rpl32 片段清楚地区分了整个产业链上游采集的七种大黄属植物。对于整个产业链中游和下游采集的样品，36 个商业饮片样品中有 25%被鉴定为掺杂物，而 8 个 TCPM 样品均来自正品大黄根茎。
结论
本研究表明，DNA 条形码是一种强大且合适的技术，可应用于整个产业链中 TCM 的追溯，从而保证临床用药安全。
Zotero 插件