Ozaki Makoto, Shimotsuma Motoshi, Hirose Tsunehisa
Nacalai Tesque, Inc., Ishibashi Kaide-cho, Muko-shi, Kyoto, 617-0004, Japan.
Nacalai Tesque, Inc., Ishibashi Kaide-cho, Muko-shi, Kyoto, 617-0004, Japan.
Anal Biochem. 2022 Oct 15;655:114837. doi: 10.1016/j.ab.2022.114837. Epub 2022 Aug 8.
Nicotinamide adenine dinucleotide, a coenzyme involved in the activation of sirtuins, contributes to various regulations in vivo. However, highly hydrophilic nicotinamide metabolites are difficult to separate by high-performance liquid chromatography (HPLC) using octadecyl (C) columns, which operate via hydrophobic interaction. PBr columns packed with silica gel modified with the pentabromobenzyl group having strong dispersion forces show good retention ability for various highly hydrophilic compounds. Additionally, the peak shape obtained with the PBr column did not collapse like that of the HILIC column, even when a large amount of water was injected. Separation of 11 highly hydrophilic nicotinamide metabolites using a PBr column under simple conditions resulted in baseline separation, but separation on a C column was not complete. The peak shape for each compound was better than that in previous studies. Furthermore, the separation of nicotinamide metabolites in tomato using a PBr column enable a more sensitive detection than that using a C column. SUBJECT CATEGORY: Chromatographic Technique.
烟酰胺腺嘌呤二核苷酸是一种参与沉默调节蛋白激活的辅酶，在体内发挥多种调节作用。然而，高亲水性的烟酰胺代谢物很难通过使用十八烷基（C）柱的高效液相色谱（HPLC）进行分离，这种柱是通过疏水相互作用来运行的。填充有经五溴苄基修饰的硅胶且具有强色散力的PBr柱，对各种高亲水性化合物表现出良好的保留能力。此外，即使注入大量水，使用PBr柱获得的峰形也不会像亲水作用色谱柱那样塌陷。在简单条件下使用PBr柱分离11种高亲水性烟酰胺代谢物实现了基线分离，但在C柱上的分离并不完全。每种化合物的峰形都比以前的研究更好。此外，使用PBr柱分离番茄中的烟酰胺代谢物比使用C柱能实现更灵敏的检测。主题类别：色谱技术。
