Department of Animal Science, College of agriculture & natural resource, University of Tehran, Karaj, Iran.
Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Acta Trop. 2022 Nov;235:106646. doi: 10.1016/j.actatropica.2022.106646. Epub 2022 Aug 8.
Since the first detection of canine circovirus (CanineCV), several reports have been published over the last decade about the worldwide distribution of this emerging virus of dogs. In order to investigate the prevalence and genomic features of CanineCV in Iranian dogs, a total of 203 dog faecal samples was collected between February and November 2018 from five different geographical regions and screened by real-time PCR (qPCR). Thirteen dogs (6.4%) tested positive for CanineCV DNA, all being detected in co-infections with the highly virulent canine parvovirus (CPV). Three partial replicase nucleotide sequences of the detected CanineCV strains were obtained and compared with the reference sequences deposited in the GenBank database. The Iranian CanineCV sequences had a nucleotide identity of 96.4-98.2% each to other and of 88.3-98.2% with other sequences available on the GenBank. Phylogenetic analysis showed that the Iranian sequences are more closely related to Turkish strains than to strains reported from other countries. The present study provides new insights into the CanineCV molecular epidemiology and its possible role as a co-infectious pathogen.
自犬圆环病毒（CanineCV）首次被检测到以来，在过去十年中，已有数篇关于这种新兴犬病毒在全球分布的报告。为了调查伊朗犬中环病毒的流行情况和基因组特征，我们于 2018 年 2 月至 11 月从五个不同地理区域采集了 203 份犬粪便样本，并通过实时 PCR（qPCR）进行了筛查。检测到的犬圆环病毒 DNA 在 13 只犬（6.4%）中呈阳性，均与高度毒力的犬细小病毒（CPV）同时感染。获得了三个检测到的犬圆环病毒株的部分复制酶核苷酸序列，并与 GenBank 数据库中已提交的参考序列进行了比较。伊朗犬圆环病毒序列彼此之间的核苷酸同一性为 96.4-98.2%，与 GenBank 上其他序列的核苷酸同一性为 88.3-98.2%。系统发育分析表明，与其他国家报道的毒株相比，伊朗毒株与土耳其毒株的亲缘关系更为密切。本研究为犬圆环病毒的分子流行病学及其作为协同感染性病原体的可能作用提供了新的见解。
