Shang Peng, Zhang Bo, Li Pan, Ahmed Zulfiqar, Hu Xiaoxiang, Chamba Yangzom, Zhang Hao
Laboratory National Engineering for Animal Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing 100193, China.
Department of animal husbandry, College of Animal Science, Tibet Agriculture and Animal Husbandry College, Linzhi 860000, China.
Animals (Basel). 2022 Jul 27;12(15):1919. doi: 10.3390/ani12151919.
Elevated environments such as plateaus are often classified as low oxygen environments. The hypoxic adaptation mechanisms utilized by organisms in these conditions are not well understood. To address this, the differentially expressed genes (DEGs) involved in hypoxia adaptation were assessed using two pig breeds (Tibetan pig [TP] and Yorkshire sow [YY]). Genes related to lung tissue responses to hypoxia were assessed using transcriptomic (using RNA-seq) and proteomic (using iTRAQ) analysis. A total of 1021 DEGs were screened out. In the iTRAQ omics data, a total of 22,100 peptides were obtained and 4518 proteins were found after filtering. A total of 271 differentially expressed proteins [DEPs] were screened using the conditions of p < 0.05; FC ≤ 0.833; and FC ≥ 1.2. A total of 14 DEGs at the mRNA and protein levels were identified and found to be associated with regulation of the inflammatory response; blood particles; and MAPK cascade response regulation. Among the DEGs, six were associated with hypoxia adaptation function (mitochondria and glycolysis) in pigs. The results of this study identify novel candidate genes involved in porcine hypoxia adaptation mechanisms.
诸如高原等海拔较高的环境通常被归类为低氧环境。目前对于生物在这些条件下所利用的低氧适应机制还了解甚少。为了解决这个问题，利用两个猪品种（藏猪[TP]和大白母猪[YY]）评估了参与低氧适应的差异表达基因（DEG）。使用转录组学（RNA测序）和蛋白质组学（iTRAQ）分析评估了与肺组织对低氧反应相关的基因。共筛选出1021个DEG。在iTRAQ组学数据中，共获得22100个肽段，过滤后发现4518种蛋白质。使用p < 0.05、FC ≤ 0.833和FC ≥ 1.2的条件筛选出271种差异表达蛋白[DEP]。在mRNA和蛋白质水平上共鉴定出14个DEG，发现它们与炎症反应调节、血液颗粒和丝裂原活化蛋白激酶（MAPK）级联反应调节有关。在这些DEG中，有6个与猪的低氧适应功能（线粒体和糖酵解）相关。本研究结果确定了参与猪低氧适应机制的新候选基因。
