Yin Yikai, Zhang Jian, Li Xindi, Duan Mengqi, Zhao Mingxuan, Zhang Feifan, Chamba Yangzom, Shang Peng
College of Animal Science, Tibet Agriculture and Animal Husbandry College, Linzhi 860000, China.
The Provincial and Ministerial Co-Founded Collaborative Innovation Center for R&D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Linzhi 860000, China.
Vet Sci. 2024 Jun 21;11(7):283. doi: 10.3390/vetsci11070283.
The purpose of this study was to explore and verify genes that regulate the reproductive traits of Tibetan pigs at the mRNA level. The ovarian tissues of Tibetan pigs (TPs) and Yorkshire pigs (YPs) were selected as research objects, and cDNA libraries of the ovarian tissue transcripts of Tibetan pigs and Yorkshire pigs were successfully constructed by the RNA-Seq technique. A total of 651 differentially expressed genes (DEGs) were screened, including 414 up-regulated genes and 237 down-regulated genes. Through GO and KEGG enrichment analysis, it was found that these differentially expressed genes were significantly enriched in cell process, reproductive process, reproduction, cell proliferation, binding, and catalytic activity, as well as oxidative phosphorylation, endocrine resistance, thyroid hormone, Notch, and other signal transduction pathways. Genes significantly enriched in pathways closely related to reproductive regulation were analyzed and selected, and the , , , , , , , , , , , and genes were identified as important candidate genes. Twelve differentially expressed genes related to reproduction were verified by RT-qPCR. The results showed that the expression of the , , , , , and genes was significantly higher in Tibetan pigs than in Yorkshire pigs, while the expression of the , , , , and genes in Tibetan pigs was significantly lower than in Yorkshire pigs. The purpose of this study is to provide a theoretical basis for exploring the molecular mechanism of reproductive trait effect genes and the application of molecular breeding in Tibetan pigs.
本研究的目的是在mRNA水平上探索和验证调控藏猪繁殖性状的基因。选取藏猪（TPs）和大白猪（YPs）的卵巢组织作为研究对象，采用RNA-Seq技术成功构建了藏猪和大白猪卵巢组织转录本的cDNA文库。共筛选出651个差异表达基因（DEGs），其中上调基因414个，下调基因237个。通过GO和KEGG富集分析发现，这些差异表达基因显著富集于细胞过程、生殖过程、繁殖、细胞增殖、结合和催化活性，以及氧化磷酸化、内分泌抵抗、甲状腺激素、Notch等信号转导通路。对在与生殖调控密切相关的通路中显著富集的基因进行分析和筛选，确定了 、 、 、 、 、 、 、 、 、 、 和 基因作为重要候选基因。通过RT-qPCR验证了12个与繁殖相关的差异表达基因。结果表明， 、 、 、 、 和 基因在藏猪中的表达显著高于大白猪，而 、 、 、 和 基因在藏猪中的表达显著低于大白猪。本研究旨在为探索繁殖性状效应基因的分子机制及分子育种在藏猪中的应用提供理论依据。
