Iacono Eleonora, Lanci Aliai, Gugole Penelope, Merlo Barbara
Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia, 40064 Bologna, Italy.
Interdepartmental Centre for Industrial Research in Health Sciences and Technologies, University of Bologna, Via Tolara di Sopra 41/E, Ozzano Emilia, 40064 Bologna, Italy.
Animals (Basel). 2022 Aug 3;12(15):1967. doi: 10.3390/ani12151967.
To use Mesenchymal Stromal Cells (MSCs) in equine patients, isolation and expansion are performed in a laboratory. Cells are then sent back to the veterinary clinic. The main goal of storage conditions during cell transport is to preserve their biological properties and viability. The aim of this study was to evaluate the effects of storage solutions, temperature and time on the characteristics of equine adipose tissue and Wharton's jelly-derived MSCs. We compared two different storage solutions (plasma and 0.9% NaCl), two different temperatures (4 °C and room temperature) and three time frames (6, 24, 48 h). Cell viability, colony-forming units, trilineage differentiation, the expression of CD45 and CD90 antigens and adhesion potentials were evaluated. Despite the molecular characterization and differentiation potential were not influenced by storage conditions, viability, colony-forming units and adhesion potential are influenced in different way, depending on MSCs sources. Overall, this study found that, despite equine adipose tissue MSCs being usable after 24 h of storage, cells derived from Wharton's jelly need to be used within 6 h. Moreover, while for adipose cells the best conservation solutions seems to be plasma, the cell viability of Wharton's jelly MSCs declined in both saline and plasma solution, confirming their reduced resistance to conservation.
为了在马属动物患者中使用间充质基质细胞（MSCs），需在实验室中进行分离和扩增。然后将细胞送回兽医诊所。细胞运输过程中储存条件的主要目标是保持其生物学特性和活力。本研究的目的是评估储存溶液、温度和时间对马脂肪组织和脐带华通氏胶来源的MSCs特性的影响。我们比较了两种不同的储存溶液（血浆和0.9%氯化钠）、两种不同的温度（4℃和室温）以及三个时间框架（6、24、48小时）。评估了细胞活力、集落形成单位、三系分化、CD45和CD90抗原的表达以及黏附潜能。尽管分子特征和分化潜能不受储存条件影响，但活力、集落形成单位和黏附潜能会因MSCs来源的不同而受到不同程度的影响。总体而言，本研究发现，尽管马脂肪组织来源的MSCs在储存24小时后仍可使用，但脐带华通氏胶来源的细胞需要在6小时内使用。此外，虽然对于脂肪细胞来说，最佳的保存溶液似乎是血浆，但脐带华通氏胶MSCs在盐水和血浆溶液中的细胞活力均下降，证实了它们对保存的抵抗力较低。
