Jagelaviciute Jolita, Basinskiene Loreta, Cizeikiene Dalia, Syrpas Michail
Department of Food Science and Technology, Kaunas University of Technology, Radvilenu Rd. 19, LT-50254 Kaunas, Lithuania.
Bioprocess Research Centre, Kaunas University of Technology, Radvilenu Rd. 19, LT-50254 Kaunas, Lithuania.
Foods. 2022 Aug 3;11(15):2321. doi: 10.3390/foods11152321.
Cranberry pomace obtained after juice production is a good source of dietary fiber and other bioactive compounds. In this study, cranberry pomace was hydrolyzed with Viscozyme L, Pectinex Ultra Tropical, Pectinex Yieldmash Plus, and Celluclast 1.5L (Novozyme A/S, Denmark). The soluble and insoluble dietary fiber was determined using the Megazyme kit, while the changes in mono-, disaccharide and oligosaccharides' contents were determined using HPLC-RI; the total phenolic contents were determined by Folin-Ciocalteu's Assay. Prebiotic activity, using two probiotic strains DSM 20079 and DSM 20105, was investigated. The technological properties, such as hydration and oil retention capacity, were evaluated. The enzymatic treatment increased the yield of short-chain soluble saccharides. The highest oligosaccharide content was obtained using Viscozyme L and Pectinex Ultra Tropical. All of the tested extracts of cranberry pomace showed the ability to promote growth of selected probiotic bacteria. The insoluble dietary fiber content decreased in all of the samples, while the soluble dietary fiber increased just in samples hydrolyzed with Celluclast 1.5L. The highest content of total phenolic compounds was obtained using Viscozyme L and Pectinex Ultra Tropical (10.9% and 13.1% higher than control, respectively). The enzymatically treated cranberry pomace exhibited lower oil and water retention capacities in most cases. In contrast, water swelling capacity increased by 23% and 70% in samples treated with Viscozyme L and Celluclast 1.5L, respectively. Enzymatically treated cranberry pomace has a different composition and technological properties depending on the enzyme used for hydrolysis and can be used in various novel food products.
果汁生产后得到的蔓越莓果渣是膳食纤维和其他生物活性化合物的良好来源。在本研究中,使用里氏木霉复合酶(Viscozyme L)、热带果胶酶(Pectinex Ultra Tropical)、高产果胶酶(Pectinex Yieldmash Plus)和1.5L纤维素酶(Celluclast 1.5L,丹麦诺维信公司)对蔓越莓果渣进行水解。使用Megazyme试剂盒测定可溶性和不溶性膳食纤维,同时使用高效液相色谱-示差折光检测器(HPLC-RI)测定单糖、双糖和寡糖含量的变化;通过福林-酚法测定总酚含量。使用两株益生菌DSM 20079和DSM 20105研究益生元活性。评估了水合能力和持油能力等工艺性能。酶处理提高了短链可溶性糖类的产量。使用里氏木霉复合酶和热带果胶酶获得了最高的寡糖含量。所有测试的蔓越莓果渣提取物均显示出促进所选益生菌生长的能力。所有样品中的不溶性膳食纤维含量均下降,而仅用1.5L纤维素酶水解的样品中可溶性膳食纤维增加。使用里氏木霉复合酶和热带果胶酶获得了最高的总酚化合物含量(分别比对照高10.9%和13.1%)。在大多数情况下,酶处理的蔓越莓果渣表现出较低的持油和持水能力。相比之下,用里氏木霉复合酶和1.5L纤维素酶处理的样品的水溶胀能力分别提高了23%和70%。根据用于水解的酶不同,酶处理的蔓越莓果渣具有不同的组成和工艺性能,可用于各种新型食品中。