Syrpas Michail, Valanciene Egle, Augustiniene Ernesta, Malys Naglis
Department of Food Science & Technology, Faculty of Chemical Technology, Kaunas University of Technology, Radvilėnų Pl. 19, LT-50254 Kaunas, Lithuania.
Bioprocess Research Centre, Faculty of Chemical Technology, Kaunas University of Technology, Radvilėnų Pl. 19, LT-50254 Kaunas, Lithuania.
Antioxidants (Basel). 2021 May 13;10(5):773. doi: 10.3390/antiox10050773.
Bilberry ( L.) pomace contains a significant amount of polyphenols and can serve as a basis for food additives, nutraceuticals, and functional foods. Although various techniques can be employed to recover bioactive fractions from berry pomaces, data on enzyme-assisted extraction (EAE) of bilberry pomace are rather scarce. This study aimed to optimize critical EAE parameters using Viscozyme L to obtain a high-yield extract with enhanced antioxidant capacity. Central composite design and response surface methodology evaluating the effect of four independent variables, namely, pH, temperature, extraction time, and enzyme concentration on three responses, were employed to define optimal EAE conditions. Under the optimal conditions (pH: 4.5, temperature 46 °C, 1 h of extraction, and 2 active units (AU) of Viscozyme L/g of pomace), EAE yielded 56.15 g/100 g DW of the water-soluble fraction. Comparison with conventional maceration indicated that EAE, besides the yield, significantly increased the in vitro antioxidant capacity measured by the total phenolic content, ABTS, ORAC, and CUPRAC assays. Moreover, an increase was observed for the measured mono- and disaccharide as well as anthocyanin content. Overall, this study demonstrates the improved efficiency of EAE over conventional solid-liquid extraction to recover fractions with a higher yield and enhanced functional properties in a fast and sustainable manner.
欧洲越橘（L.）果渣含有大量多酚，可作为食品添加剂、营养保健品和功能性食品的基础原料。尽管可以采用多种技术从浆果渣中回收生物活性成分，但关于欧洲越橘果渣酶辅助提取（EAE）的数据却相当匮乏。本研究旨在使用Viscozyme L优化关键的EAE参数，以获得具有增强抗氧化能力的高产提取物。采用中心复合设计和响应面方法，评估四个自变量（即pH值、温度、提取时间和酶浓度）对三个响应的影响，以确定最佳的EAE条件。在最佳条件下（pH值：4.5，温度46℃，提取1小时，每克果渣使用2个活性单位（AU）的Viscozyme L），EAE得到了56.15 g/100 g干重的水溶性部分。与传统浸渍法相比，EAE除了提高产量外，还显著提高了通过总酚含量、ABTS、ORAC和CUPRAC测定法测得的体外抗氧化能力。此外，单糖、双糖以及花青素含量也有所增加。总体而言，本研究表明，与传统固液萃取相比，EAE具有更高的效率，能够以快速且可持续的方式回收产量更高、功能特性更强的成分。
