Max-Planck-Institute for Polymer Research, Ackermannweg 10, 55122 Mainz, Germany.
Department of Dermatology, University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstraße 1, 55131 Mainz, Germany.
Cells. 2022 Jul 28;11(15):2323. doi: 10.3390/cells11152323.
Cell therapy is an important new method in medicine and is being used for the treatment of an increasing number of diseases. The challenge here is the precise tracking of cells in the body and their visualization. One method to visualize cells more easily with current methods is their labeling with nanoparticles before injection. However, for a safe and sufficient cell labeling, the nanoparticles need to remain in the cell and not be exocytosed. Here, we test a glucose-PEG-coated gold nanoparticle for the use of such a cell labeling. To this end, we investigated the nanoparticle exocytosis behavior from PLX-PAD cells, a cell type currently in clinical trials as a potential therapeutic agent. We showed that the amount of exocytosed gold from the cells was influenced by the uptake time and loading amount. This observation will facilitate the safe labeling of cells with nanoparticles in the future and contribute to stem cell therapy research.
细胞治疗是医学中的一种重要新方法，正被用于越来越多疾病的治疗。这里的挑战是在体内精确追踪细胞并对其进行可视化。目前，一种更轻松地对细胞进行可视化的方法是在注射前用纳米颗粒对其进行标记。然而，为了安全且充分地对细胞进行标记，纳米颗粒需要留在细胞内而不被外排。在这里，我们测试了一种葡萄糖-聚乙二醇（PEG）包覆的金纳米颗粒，以用于这种细胞标记。为此，我们研究了 PLX-PAD 细胞的纳米颗粒外排行为，PLX-PAD 细胞是目前临床试验中作为潜在治疗剂的一种细胞类型。我们表明，细胞中外排的金的量受到摄取时间和加载量的影响。这一观察结果将有助于未来安全地用纳米颗粒对细胞进行标记，并为干细胞治疗研究做出贡献。
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